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Literature summary extracted from

  • Rearick, J.I.; Sadler, J.E.; Paulson, J.C.; Hill, R.L.
    Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland (1979), J. Biol. Chem., 254, 4444-4451 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.4 0.2
-
 beta-D-Gal-(1->3)-beta-D-GalNAc pH 6.5, 37°C, enzyme form A Sus scrofa
2.4.3.4 0.21
-
 beta-D-Gal-(1->3)-beta-D-GalNAc pH 6.5, 37°C, enzyme form B Sus scrofa
2.4.3.4 0.35
-
 [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine pH 6.5, 37°C, enzyme form A Sus scrofa
2.4.3.4 0.39
-
 [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine pH 6.5, 37°C, enzyme form B Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.4 50000
-
 gel filtrazion, enzyme form A and B Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.2 CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R Sus scrofa
-
 CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine Sus scrofa the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine Sus scrofa the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine Sus scrofa
-
 CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine Sus scrofa the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.2 Sus scrofa
-
-
-
2.4.3.4 Sus scrofa Q02745
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.4 purification of enzyme form A and B Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.2 submandibular gland
-
 Sus scrofa
-
2.4.3.4 submandibular gland
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.2 asialo-alpha1 acid glycoprotein
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 Sus scrofa CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + agalactofetuin
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + antifreeze protein
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + asialotransferrin
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R 100% activity Sus scrofa CMP + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-beta-D-galactosyl-(1->3)-beta-D-glucosyl-(1->4)-R 2.9% activity compared to beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R 1% activity compared to beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R Sus scrofa CMP + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GD1a very low activity Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GM1
-
 Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GM2 very low activity Sus scrofa ?
-
 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + porcine submaxillary asialo-mucin
-
 Sus scrofa ?
-
 ?
2.4.3.2 additional information no activity with beta-D-galactosyl-(1->6)-N-acetyl-beta-D-galactosaminyl-R, beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosyl-R, beta-D-galactosyl-(1->6)-N-acetyl-beta-D-glucosyl-R, beta-D-galactosyl-(1->4)beta-D-glucosyl-R, beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-beta-D-galactosyl-(1->3)-beta-D-glucosyl-(1->4)-R, D-galactose, and methyl-beta-D-galactopyranoside. Ganglioside GL-4, lactosyl ceramide do not serve as acceptors Sus scrofa ?
-
-
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc Sus scrofa CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 Sus scrofa CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc Sus scrofa CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
 Sus scrofa CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
 ?
2.4.3.4 CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc Sus scrofa CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
 ?
2.4.3.4 CMP-N-acetylneuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc best acceptor substrate Sus scrofa CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
 ?
2.4.3.4 additional information with glycoprotein and ganglioside acceptors the substrate specificity is strict, with the sequence beta-D-Gal-(1->3)-alpha-D-GalNAc serving as the exclusive acceptor. The enzyme has an equilibrium random order mechanism Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.2 beta-D-galactoside alpha2->3 sialyltransferase
-
 Sus scrofa
2.4.3.2 sialyltransferase B
-
 Sus scrofa
2.4.3.4 beta-D-galactoside alpha2->3 sialyltransferase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.4 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.4 6.5
-
 assay at Sus scrofa

General Information

EC Number General Information Comment Organism
2.4.3.4 metabolism the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc Sus scrofa