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Literature summary extracted from

  • Briggs, G.; Bulley, J.; Dickson, P.
    Catalytic domain surface residues mediating catecholamine inhibition in tyrosine hydroxylase (2014), J. Biochem., 155, 183-193 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.2 A297L the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor Rattus norvegicus
1.14.16.2 D299N inactive Rattus norvegicus
1.14.16.2 D361N the mutant shows increased Vmax compared to the wild type enzyme Rattus norvegicus
1.14.16.2 E362Q the mutant shows reduced Vmax compared to the wild type enzyme Rattus norvegicus
1.14.16.2 E362R/E365R the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor Rattus norvegicus
1.14.16.2 E365Q the mutant shows reduced Vmax compared to the wild type enzyme Rattus norvegicus
1.14.16.2 K170E/L480A inactive Rattus norvegicus
1.14.16.2 K366L the mutant shows reduced Vmax compared to the wild type enzyme Rattus norvegicus
1.14.16.2 R298L inactive Rattus norvegicus
1.14.16.2 R37E/R38E the Km value for tetrahydrobiopterin measured for the mutant is approximately half that of the wild type enzyme Rattus norvegicus
1.14.16.2 S368A the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 dopamine 1.8% residual activity at 0.01 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 300000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus
-
 L-dopa + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus P04177
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.2 phosphoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus L-dopa + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 homotetramer 4 * 70000, calculated from amino acid sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.16.2 0.000595
-
 pH and temperature not specified in the publication Rattus norvegicus dopamine