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Literature summary extracted from

  • Awano, T.; Wilming, A.; Tomita, H.; Yokooji, Y.; Fukui, T.; Imanaka, T.; Atomia, H.
    Characterization of two members among the five ADP-forming acyl coenzyme a (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis (2014), J. Bacteriol., 196, 140-147 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.13 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.0056
-
 CoA at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 0.0556
-
 ATP at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 0.132
-
 Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 0.322
-
 thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 0.329
-
 phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 1.5
-
 acetate at pH 6.5 and 55°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.13 ATP + acetate + CoA Thermococcus kodakarensis
-
 ADP + phosphate + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Thermococcus kodakarensis Q5JIA8 and Q5JIA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13 Resource Q column chromatography and Superdex 200 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ATP + 2-methylbutyrate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + 2-methylbutyryl-CoA
-
 ?
6.2.1.13 ATP + 3-methylthiopropionate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + 3-methylthiopropionyl-CoA
-
 ?
6.2.1.13 ATP + acetate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + acetyl-CoA
-
 ?
6.2.1.13 ATP + isobutyrate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + isobutyryl-CoA
-
 ?
6.2.1.13 ATP + isovalerate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + isovaleryl-CoA
-
 ?
6.2.1.13 ATP + phenylacetate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + phenylacetyl-CoA
-
 ?
6.2.1.13 ATP + thioglycolate + CoA
-
 Thermococcus kodakarensis ADP + phosphate + thioglycolyl-CoA
-
 ?
6.2.1.13 additional information almost no activity with formate, 5-aminovalerate, 4-guanidinobutyrate, 2-(imidazol-4-yl)acetate, malonate, and succinate Thermococcus kodakarensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.13 ACS III isoform Thermococcus kodakarensis
6.2.1.13 ADP-forming acyl coenzyme A synthetase
-
 Thermococcus kodakarensis
6.2.1.13 ADP-forming acyl-CoA synthetase
-
 Thermococcus kodakarensis
6.2.1.13 TK0944/TK0943 protein
-
 Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.13 26.1
-
 thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 40.6
-
 phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 49.3
-
 acetate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 50.9
-
 Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 53.1
-
 CoA at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 53.7
-
 ATP at pH 6.5 and 55°C Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.13 ATP
-
 Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.13 32.8
-
 acetate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 81
-
 thioglycolate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 123
-
 phenylacetate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 385
-
 Isobutyrate at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 965
-
 ATP at pH 6.5 and 55°C Thermococcus kodakarensis
6.2.1.13 9490
-
 CoA at pH 6.5 and 55°C Thermococcus kodakarensis