EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | additional information | NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities | Acidianus brierleyi | |
6.4.1.2 | palmitoyl-CoA | 0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities | Acidianus brierleyi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | ATP | an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
6.4.1.2 | citrate | citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM | Acidianus brierleyi | |
6.4.1.2 | Co2+ | 26% residual activity at 10-15 mM | Acidianus brierleyi | |
6.4.1.2 | CoASH | inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% | Acidianus brierleyi | |
6.4.1.2 | dithiothreitol | dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM | Acidianus brierleyi | |
6.4.1.2 | K+ | the enzyme shows less than 50% activity when the concentration is higher than 40 mM | Acidianus brierleyi | |
6.4.1.2 | malonyl-CoA | inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% | Acidianus brierleyi | |
6.4.1.2 | methylmalonyl-CoA | 60% inhibition at 0.5 mM; 79% inhibition at 0.5 mM | Acidianus brierleyi | |
6.4.1.2 | Mg2+ | an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
6.4.1.2 | Mn2+ | an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
6.4.1.2 | Na+ | the enzyme shows less than 50% activity when the concentration is higher than 40 mM | Acidianus brierleyi | |
6.4.1.2 | succinyl-CoA | 57% inhibition at 0.5 mM; 84% inhibition at 0.5 mM | Acidianus brierleyi | |
6.4.1.2 | sulfate | sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM | Acidianus brierleyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.2 | 0.04 | - |
ATP | with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C | Acidianus brierleyi | |
6.4.1.2 | 0.05 | - |
ATP | with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C | Acidianus brierleyi | |
6.4.1.2 | 0.1 | - |
propionyl-CoA | at pH 7.5 and 70°C | Acidianus brierleyi | |
6.4.1.2 | 0.17 | - |
acetyl-CoA | at pH 7.5 and 70°C | Acidianus brierleyi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | Mg2+ | required, maximum activity at 4 mM | Acidianus brierleyi | |
6.4.1.2 | additional information | Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity | Acidianus brierleyi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 540000 | - |
gel filtration | Acidianus brierleyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Acidianus brierleyi | - |
ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + propionyl-CoA + HCO3- | Acidianus brierleyi | - |
ADP + phosphate + methylmalonyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.2 | Acidianus brierleyi | Q877I4 and Q877I5 and Q877I3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.2 | Streptavidin column chromatography and Superose 6 gel filtration | Acidianus brierleyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Acidianus brierleyi | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + propionyl-CoA + HCO3- | - |
Acidianus brierleyi | ADP + phosphate + methylmalonyl-CoA | - |
? | |
6.4.1.2 | additional information | neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme | Acidianus brierleyi | ? | - |
? | |
6.4.1.2 | n-butyryl-CoA + HCO3- | 4.6% activity compared to acetyl-CoA or propionyl-CoA | Acidianus brierleyi | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.2 | homotrimer | 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.4.1.2 | ACCB | - |
Acidianus brierleyi |
6.4.1.2 | AccC | - |
Acidianus brierleyi |
6.4.1.2 | acyl coenzyme A carboxylase | - |
Acidianus brierleyi |
6.4.1.2 | acyl-CoA carboxylase | - |
Acidianus brierleyi |
6.4.1.2 | PccB | - |
Acidianus brierleyi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 60 | 70 | - |
Acidianus brierleyi |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 50 | 90 | the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above | Acidianus brierleyi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 6.4 | 6.9 | - |
Acidianus brierleyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | ATP | the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP | Acidianus brierleyi |