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Literature summary extracted from

  • Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.
    Characterization of a bifunctional archaeal acyl coenzyme A carboxylase (2003), J. Bacteriol., 185, 938-947 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 additional information NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities Acidianus brierleyi
6.4.1.2 palmitoyl-CoA 0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities Acidianus brierleyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ATP an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
6.4.1.2 citrate citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM Acidianus brierleyi
6.4.1.2 Co2+ 26% residual activity at 10-15 mM Acidianus brierleyi
6.4.1.2 CoASH inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% Acidianus brierleyi
6.4.1.2 dithiothreitol dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM Acidianus brierleyi
6.4.1.2 K+ the enzyme shows less than 50% activity when the concentration is higher than 40 mM Acidianus brierleyi
6.4.1.2 malonyl-CoA inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% Acidianus brierleyi
6.4.1.2 methylmalonyl-CoA 60% inhibition at 0.5 mM; 79% inhibition at 0.5 mM Acidianus brierleyi
6.4.1.2 Mg2+ an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
6.4.1.2 Mn2+ an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities Acidianus brierleyi
6.4.1.2 Na+ the enzyme shows less than 50% activity when the concentration is higher than 40 mM Acidianus brierleyi
6.4.1.2 succinyl-CoA 57% inhibition at 0.5 mM; 84% inhibition at 0.5 mM Acidianus brierleyi
6.4.1.2 sulfate sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM Acidianus brierleyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.04
-
ATP with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C Acidianus brierleyi
6.4.1.2 0.05
-
ATP with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C Acidianus brierleyi
6.4.1.2 0.1
-
propionyl-CoA at pH 7.5 and 70°C Acidianus brierleyi
6.4.1.2 0.17
-
acetyl-CoA at pH 7.5 and 70°C Acidianus brierleyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Mg2+ required, maximum activity at 4 mM Acidianus brierleyi
6.4.1.2 additional information Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity Acidianus brierleyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 540000
-
gel filtration Acidianus brierleyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Acidianus brierleyi
-
ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3- Acidianus brierleyi
-
ADP + phosphate + methylmalonyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Acidianus brierleyi Q877I4 and Q877I5 and Q877I3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 Streptavidin column chromatography and Superose 6 gel filtration Acidianus brierleyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Acidianus brierleyi ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3-
-
Acidianus brierleyi ADP + phosphate + methylmalonyl-CoA
-
?
6.4.1.2 additional information neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme Acidianus brierleyi ?
-
?
6.4.1.2 n-butyryl-CoA + HCO3- 4.6% activity compared to acetyl-CoA or propionyl-CoA Acidianus brierleyi ?
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 homotrimer 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACCB
-
Acidianus brierleyi
6.4.1.2 AccC
-
Acidianus brierleyi
6.4.1.2 acyl coenzyme A carboxylase
-
Acidianus brierleyi
6.4.1.2 acyl-CoA carboxylase
-
Acidianus brierleyi
6.4.1.2 PccB
-
Acidianus brierleyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 60 70
-
Acidianus brierleyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.2 50 90 the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above Acidianus brierleyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 6.4 6.9
-
Acidianus brierleyi

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 ATP the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP Acidianus brierleyi