Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ushimaru, R.; Ruszczycky, M.W.; Chang, W.C.; Yan, F.; Liu, Y.N.; Liu, H.W.
    Substrate conformation correlates with the outcome of hyoscyamine 6beta-hydroxylase catalyzed oxidation reactions (2018), J. Am. Chem. Soc., 140, 7433-7436 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.13 expression in Escherichia coli Hyoscyamus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.13 Iron
-
 Hyoscyamus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.13 Hyoscyamus niger
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.13 (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus niger scopolamine + succinate + CO2 + H2O
-
 ?
1.14.20.13 additional information enzyme is able to catalyze hydroxylation, dehydrogenation and in vitro epoxidation reactions all giving scopolamine. Hydroxyl rebound is unlikely to take place during the cyclization reaction and the hydroxylase versus oxidative cyclase activity is correlated with the presence of an exo-hydroxy group having syn-periplanar geometry with respect to the adjacent H atom to be abstracted Hyoscyamus niger ?
-
 ?