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Literature summary extracted from

  • Palma-Orozco, G.; Marrufo-Hernandez, N.A.; Sampedro, J.G.; Najera, H.
    Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila) (2014), J. Agric. Food Chem., 62, 9832-9840 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 ascorbic acid complete inhibition at 0.1 mM Mangifera indica
1.14.18.1 benzoic acid 31% inhibition at 0.1 mM, 52% inhibition at 10 mM Mangifera indica
1.14.18.1 Citric acid 30% inhibition at 0.1 mM, 33% inhibition at 10 mM Mangifera indica
1.14.18.1 CuSO4 87% inhibition at 0.1 mM, 50% inhibition at 10 mM Mangifera indica
1.14.18.1 EDTA 10% inhibition at 0.1 mM, 21% inhibition at 10 mM Mangifera indica
1.14.18.1 kojic acid 48% inhibition at 0.1 mM, complete inhibition at 10 mM Mangifera indica
1.14.18.1 MgCl2 10% inhibition at 0.1-1.0 mM, 11% inhibition at 10 mM Mangifera indica
1.14.18.1 NaCl 55% inhibition at 0.1 mM, 59% inhibition at 10 mM Mangifera indica
1.14.18.1 potassium sorbate 23% inhibition at 0.1 mM, 37% inhibition at 10 mM Mangifera indica
1.14.18.1 Sodium metabisulfite complete inhibition at 0.1 mM Mangifera indica
1.14.18.1 succinic acid 18% inhibition at 0.1 mM, 31% inhibition at 10 mM Mangifera indica
1.14.18.1 tropolone complete inhibition at 0.1 mM Mangifera indica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2.77
-
 pyrogallol pH 7.0, 25°C Mangifera indica
1.14.18.1 3.14
-
 4-methylcatechol pH 7.0, 25°C Mangifera indica
1.14.18.1 15.14
-
 catechol pH 7.0, 25°C Mangifera indica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 CaCl2 68% activation at 0.1 mM, 97% activation at 1 mM Mangifera indica
1.14.18.1 KCl 13% activation at 0.1 mM, 20% activation at 10 mM Mangifera indica
1.14.18.1 MgSO4 23% activation at 0.1 mM, 49% activation at 10 mM Mangifera indica
1.14.18.1 MnSO4 77% activation at 10 mM Mangifera indica
1.14.18.1 Na2SO4 95% activation at 10 mM Mangifera indica
1.14.18.1 ZnSO4 14% activation at 0.1 mM, 82% activation at 10 mM Mangifera indica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 65000
-
 gel filtration Mangifera indica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 4-methylcatechol + O2 Mangifera indica
-
 ?
-
 ?
1.14.18.1 catechol + O2 Mangifera indica
-
 ?
-
 ?
1.14.18.1 pyrogallol + O2 Mangifera indica
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Mangifera indica
-
 cv. Manila
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 native enzyme from fruit 216fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, anion exchange chromatography, followed by gel filtration, to homogeneity Mangifera indica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit
-
 Mangifera indica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 8839
-
 purified native enzyme, pH 7.0, 25°C Mangifera indica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + O2
-
 Mangifera indica ?
-
 ?
1.14.18.1 catechol + O2
-
 Mangifera indica ?
-
 ?
1.14.18.1 additional information caffeic acid, ferulic acid, epicatechin, and phenol are no substrates Mangifera indica ?
-
 ?
1.14.18.1 pyrogallol + O2
-
 Mangifera indica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 dimer 2 * 31500, SDS-PAGE Mangifera indica

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
-
 Mangifera indica
1.14.18.1 PPO
-
 Mangifera indica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 65
-
-
 Mangifera indica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 20 70 activity range, inactivation at 80°C Mangifera indica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 20 70 stable at Mangifera indica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6
-
-
 Mangifera indica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 3 7 activity range, drastic decrease in activity at pH 7.5 and above Mangifera indica