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Literature summary extracted from

  • Wang, L.; Li, Z.; Zhu, M.; Meng, L.; Wang, H.; Ng, T.B.
    An acidic feruloyl esterase from the mushroom Lactarius hatsudake A potential animal feed supplement (2016), Int. J. Biol. Macromol., 93, 290-295 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Al3+ 5 mM, 90% residual activity Lactarius hatsudake
3.1.1.73 Ca2+ 5 mM, 91% residual activity Lactarius hatsudake
3.1.1.73 Cd2+ 5 mM, 84% residual activity Lactarius hatsudake
3.1.1.73 Cu2+ 5 mM, 88% residual activity Lactarius hatsudake
3.1.1.73 Fe2+ 5 mM, no residual activity Lactarius hatsudake
3.1.1.73 Hg2+ 5 mM, no resiudal activity Lactarius hatsudake
3.1.1.73 Mn2+ 5 mM, 83% residual activity Lactarius hatsudake
3.1.1.73 additional information not inhibiting/activating: K+ or Zn2+ at 5 mM Lactarius hatsudake

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Mg2+ 5 mM, 114% of initial activity Lactarius hatsudake

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 55000
-
 gel filtration Lactarius hatsudake

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Lactarius hatsudake
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Lactarius hatsudake

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 5
-
 substrate methyl p-coumarate, pH 4.0, 30°C Lactarius hatsudake
3.1.1.73 9.66
-
 substrate methyl ferulate, pH 4.0, 30°C Lactarius hatsudake
3.1.1.73 13.14
-
 substrate methyl caffeate, pH 4.0, 30°C Lactarius hatsudake

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 destarched wheat bran + H2O
-
 Lactarius hatsudake ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Lactarius hatsudake caffeate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Lactarius hatsudake ferulate + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Lactarius hatsudake methanol + p-coumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 55000, SDS-PAGE Lactarius hatsudake

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30
-
-
 Lactarius hatsudake

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 70
-
 66% of maximum activity Lactarius hatsudake

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 4
-
-
 Lactarius hatsudake

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3
-
 71% of maximum activity Lactarius hatsudake
3.1.1.73 6
-
 completely inactive Lactarius hatsudake