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Literature summary extracted from

  • Phadtare, P.; Joshi, S.; Satyanarayana, T.
    Recombinant thermo-alkali-stable endoglucanase of Myceliopthora thermophila BJA (rMt-egl) Biochemical characteristics and applicability in enzymatic saccharification of agro-residues (2017), Int. J. Biol. Macromol., 104, 107-116 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation enzyme releases high amounts of reducing sugars from wheat bran and corn cobs, being a useful biocatalyst for producing bioethanol and fine chemicals from agroresidues Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Pichia pastoris Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 homology modeling of structure. Residues E234 and E344 display a catalytically important role Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+ 1 mM, 62% residual activity Thermothelomyces thermophilus
3.2.1.4 EDTA 1 mM, 66% residual activity Thermothelomyces thermophilus
3.2.1.4 Fe3+ 1 mM, 62% residual activity Thermothelomyces thermophilus
3.2.1.4 Mg2+ 1 mM, 54% residual activity Thermothelomyces thermophilus
3.2.1.4 Pb2+ 1 mM, 30% residual activity Thermothelomyces thermophilus
3.2.1.4 phenylmethylsulfonyl fluoride 1 mM, 60% residual activity Thermothelomyces thermophilus
3.2.1.4 SDS 0.1%, 56% residual activity Thermothelomyces thermophilus
3.2.1.4 Zn2+ 1 mM, 64% residual activity Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethylcellulose Km value 5 mg/ml, pH 10, 50°C Thermothelomyces thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 47000
-
 gel fitration Thermothelomyces thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 Acetone 5%, 75% residual activity Thermothelomyces thermophilus
3.2.1.4 Butanol 5%, 75% residual activity Thermothelomyces thermophilus
3.2.1.4 Ethanol 5%, 70% residual activity Thermothelomyces thermophilus
3.2.1.4 Glycerol 5%, 85% residual activity Thermothelomyces thermophilus
3.2.1.4 hexane 5%, 85% residual activity Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermothelomyces thermophilus
-
-
-
3.2.1.4 Thermothelomyces thermophilus BJA
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Thermothelomyces thermophilus cellobiose + cellotriose + cellotetraose + cellopentaose
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Thermothelomyces thermophilus BJA cellobiose + cellotriose + cellotetraose + cellopentaose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 47000, SDS-PAGE Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EGL
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 90
-
 half-life 60 min Thermothelomyces thermophilus
3.2.1.4 100
-
 half-life 15 min Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 1020
-
 carboxymethylcellulose pH 10, 50°C Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 10
-
-
 Thermothelomyces thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 12 3 h, about 70% residual activity Thermothelomyces thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethylcellulose kcat/Km value 204 ml/mg/s Thermothelomyces thermophilus