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Literature summary extracted from

  • Wriessnegger, T.; Moser, S.; Emmerstorfer-Augustin, A.; Leitner, E.; Mueller, M.; Kaluzna, I.; Schuermann, M.; Mink, D.; Pichler, H.
    Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions (2016), Fungal Genet. Biol., 89, 114-125 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.1 additional information the enzyme shows significantly enhanced activity upon over-expression and cultivation optimization of the DNA repair and recombination protein RAD52. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition Homo sapiens
1.14.14.99 additional information the enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition Mentha x piperita

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 overexpression in Pichia pastoris. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition Homo sapiens
1.14.14.99 overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition Mentha x piperita

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Homo sapiens P10635
-
-
1.14.14.99 Mentha x piperita Q9XHE7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.99 (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
 Mentha x piperita (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 CP2D6
-
 Homo sapiens
1.14.14.99 (-)-limonene-3-hydroxylase
-
 Mentha x piperita
1.14.14.99 PM17
-
 Mentha x piperita

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.1 cytochrome P450 a heme-thiolate protein (P-450) Homo sapiens
1.14.14.99 cytochrome P-450 a heme-thiolate protein (P-450) Mentha x piperita
1.14.14.99 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Mentha x piperita