Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mladenova, S.R.; Stein, K.R.; Bartlett, L.; Sheppard, K.
    Relaxed tRNA specificity of the Staphylococcus aureus aspartyl-tRNA synthetase enables RNA-dependent asparagine biosynthesis (2014), FEBS Lett., 588, 1808-1812 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.23 expressed in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.22 0.0004
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 0.0024
-
 tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 0.0031
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.23 ATP + L-aspartate + tRNAAsp Staphylococcus aureus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Staphylococcus aureus FPR3757
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.22 Staphylococcus aureus
-
-
-
6.1.1.23 Staphylococcus aureus
-
-
-
6.1.1.23 Staphylococcus aureus FPR3757
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.22 Ni-NTA column chromatography Staphylococcus aureus
6.1.1.23 Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Staphylococcus aureus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Staphylococcus aureus FPR3757 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 additional information no activity with tRNAAsp Staphylococcus aureus ?
-
 ?
6.1.1.22 additional information no activity with tRNAAsp Staphylococcus aureus FPR3757 ?
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
 Staphylococcus aureus AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
 Staphylococcus aureus FPR3757 AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Staphylococcus aureus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Staphylococcus aureus FPR3757 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.22 AsnRS
-
 Staphylococcus aureus
6.1.1.22 Asparaginyl-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.23 Aspartyl-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.23 AspRS
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.22 0.043
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 0.2
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 0.24
-
 tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.22 ATP
-
 Staphylococcus aureus
6.1.1.23 ATP
-
 Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.22 110
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 70
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus
6.1.1.23 100
-
 tRNAAsp at pH 7.2 and 37°C Staphylococcus aureus