BRENDA - Enzyme Database

Anthocyanidin synthase from Gerbera hybrida catalyzes the conversion of (+)-catechin to cyanidin and a novel procyanidin

Wellmann, F.; Griesser, M.; Schwab, W.; Martens, S.; Eisenreich, W.; Matern, U.; Lukacin, R.; FEBS Lett. 580, 1642-1648 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.20.4
ascorbate
maximal activity requires the addition of 0.3 mM ascorbate
Gerbera hybrid cultivar
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.20.4
expression in Saccharomyces cerevisiae
Gerbera hybrid cultivar
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.20.4
0.175
-
(+)-catechin
pH 6.0, 37°C
Gerbera hybrid cultivar
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.20.4
Fe2+
presence of ferrous iron is required
Gerbera hybrid cultivar
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.20.4
Gerbera hybrid cultivar
Q52MZ9
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.20.4
(+)-catechin + 2-oxoglutarate + O2
-
744891
Gerbera hybrid cultivar
cyanidin + succinate + CO2 + 2 H2O
-
-
-
?
1.14.20.4
additional information
product formation is essentially dependent on the presence of ferrous ions and 2-oxoglutarate. No substrates: ent(-)-catechin (2S,3R-trans-catechin), (-)-epicatechin, ent(+)-epicatechin (2S,3S-cis-epicatechin) and (-)-gallocatechin
744891
Gerbera hybrid cultivar
?
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.20.4
ascorbate
maximal activity requires the addition of 0.3 mM ascorbate
Gerbera hybrid cultivar
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.20.4
expression in Saccharomyces cerevisiae
Gerbera hybrid cultivar
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.20.4
0.175
-
(+)-catechin
pH 6.0, 37°C
Gerbera hybrid cultivar
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.20.4
Fe2+
presence of ferrous iron is required
Gerbera hybrid cultivar
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.20.4
(+)-catechin + 2-oxoglutarate + O2
-
744891
Gerbera hybrid cultivar
cyanidin + succinate + CO2 + 2 H2O
-
-
-
?
1.14.20.4
additional information
product formation is essentially dependent on the presence of ferrous ions and 2-oxoglutarate. No substrates: ent(-)-catechin (2S,3R-trans-catechin), (-)-epicatechin, ent(+)-epicatechin (2S,3S-cis-epicatechin) and (-)-gallocatechin
744891
Gerbera hybrid cultivar
?
-
-
-
-