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Literature summary extracted from

  • Lee, M.; González, J.; Robb, F.
    Extremely thermostable glutamate dehydrogenase (GDH) from the freshwater archaeon Thermococcus waiotapuensis Cloning and comparison with two marine hyperthermophilic GDHs (2002), Extremophiles, 6, 151-159 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.3
-
 Thermococcus litoralis
1.4.1.3
-
 Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.3 Ca2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 Thermococcus litoralis
1.4.1.3 Ca2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 Pyrococcus furiosus
1.4.1.3 Mg2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 1.3fold increase in activity at 10 mM CaCl2 or MgSO4 Thermococcus litoralis
1.4.1.3 Mg2+ addition of Ca2+ and Mg2+ also shows enhancement of enzyme activity. 3.5fold increase in activity at 10 mM CaCl2 or MgSO4 Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Pyrococcus furiosus P80319
-
-
1.4.1.3 Thermococcus litoralis Q56304
-
-
1.4.1.3 Thermococcus litoralis DSM 5473 Q56304
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NADP+
-
 Thermococcus litoralis 2-oxoglutarate + NH3 + NADPH + H+
-
 ?
1.4.1.3 L-glutamate + H2O + NADP+
-
 Pyrococcus furiosus 2-oxoglutarate + NH3 + NADPH + H+
-
 ?
1.4.1.3 L-glutamate + H2O + NADP+
-
 Thermococcus litoralis DSM 5473 2-oxoglutarate + NH3 + NADPH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 97
-
 specific activity increases up to 97°C Thermococcus litoralis
1.4.1.3 97
-
 specific activity increases up to 97°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 104
-
 half-life: 4.9 min in absence of KCl, 45 min in presence of 1 M KCl Thermococcus litoralis
1.4.1.3 104
-
 half-life: 67 min in absence of KCl, 562 min in presence of 1 M KCl Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 7.5
-
-
 Thermococcus litoralis
1.4.1.3 8
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.3 6.5 8 pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity Thermococcus litoralis
1.4.1.3 7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity Pyrococcus furiosus