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Literature summary extracted from

  • Fusco, F.A.; Ronca, R.; Fiorentino, G.; Pedone, E.; Contursi, P.; Bartolucci, S.; Limauro, D.
    Biochemical characterization of a thermostable endomannanase/endoglucanase from Dictyoglomus turgidum (2018), Extremophiles, 22, 131-140 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Dictyoglomus turgidum
3.2.1.78 expression in Escherichia coli Dictyoglomus turgidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 1 mM, 12% residual activity Dictyoglomus turgidum
3.2.1.78 Cu2+ 1 mM, 8% residual activity Dictyoglomus turgidum
3.2.1.78 Fe3+ 1 mM, 42% residual activity Dictyoglomus turgidum
3.2.1.78 Mg2+ 1 mM, 34% residual activity Dictyoglomus turgidum
3.2.1.78 additional information not inhibitory: EDTA, dithiothreitol Dictyoglomus turgidum
3.2.1.78 SDS 0.5%, 1% residual activity Dictyoglomus turgidum
3.2.1.78 Triton X-100 0.5%, 44% residual activity Dictyoglomus turgidum
3.2.1.78 Tween 20 0.5%, 77% residual activity Dictyoglomus turgidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethylcellulose Km value 1.83 mg/ml, pH 5.4, 70°C Dictyoglomus turgidum
3.2.1.78 additional information
-
 konjac glucomannan Km value 4.7 mg/ml, pH 5.4, 70°C Dictyoglomus turgidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 43000
-
 gel filtration Dictyoglomus turgidum
3.2.1.78 43000
-
 gel filtration Dictyoglomus turgidum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Dictyoglomus turgidum B8DZM3 bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively
-
3.2.1.4 Dictyoglomus turgidum DSM 6724 B8DZM3 bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively
-
3.2.1.78 Dictyoglomus turgidum B8DZM3 bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively
-
3.2.1.78 Dictyoglomus turgidum DSM 6724 B8DZM3 bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 215
-
 substrate konjac glucomannan, pH 5.4, 70°C Dictyoglomus turgidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Dictyoglomus turgidum ?
-
 ?
3.2.1.4 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum ?
-
 ?
3.2.1.4 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum DSM 6724 ?
-
 ?
3.2.1.78 carob glucomannan + H2O 13% of the activity with konjac glucomannan Dictyoglomus turgidum ?
-
 ?
3.2.1.78 carob glucomannan + H2O 13% of the activity with konjac glucomannan Dictyoglomus turgidum DSM 6724 ?
-
 ?
3.2.1.78 guar galactomannan + H2O 1.4% of the activity with konjac glucomannan Dictyoglomus turgidum ?
-
 ?
3.2.1.78 guar galactomannan + H2O 1.4% of the activity with konjac glucomannan Dictyoglomus turgidum DSM 6724 ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Dictyoglomus turgidum ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Dictyoglomus turgidum DSM 6724 ?
-
 ?
3.2.1.78 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum ?
-
 ?
3.2.1.78 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum DSM 6724 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 40000, SDS-PAGE, 1 * 40457, calculated Dictyoglomus turgidum
3.2.1.78 monomer 1 * 40000, SDS-PAGE, 1 * 40457, calculated Dictyoglomus turgidum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CelB
-
 Dictyoglomus turgidum
3.2.1.4 Dtur_0671
-
 Dictyoglomus turgidum
3.2.1.78 CelB
-
 Dictyoglomus turgidum
3.2.1.78 Dtur_0671
-
 Dictyoglomus turgidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 Dictyoglomus turgidum
3.2.1.78 70
-
-
 Dictyoglomus turgidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 2 h, 50% residual activtiy Dictyoglomus turgidum
3.2.1.4 88
-
 melting temperature Dictyoglomus turgidum
3.2.1.78 70
-
 2 h, 50% residual activtiy Dictyoglomus turgidum
3.2.1.78 88
-
 melting temperature Dictyoglomus turgidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.4
-
-
 Dictyoglomus turgidum
3.2.1.78 5.4
-
-
 Dictyoglomus turgidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 9 1 h, 90% residual activity Dictyoglomus turgidum
3.2.1.78 5 9 1 h, 90% residual activity Dictyoglomus turgidum