EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.1 | Sodium nitrite | - |
Methanosarcina barkeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.1 | 0.006 | - |
CoA | pH 7, 37°C | Methanosarcina barkeri | |
1.2.7.1 | 0.03 | - |
oxidized ferredoxin | clostridial ferredoxin, pH 7, 37°C | Methanosarcina barkeri | |
1.2.7.1 | 0.07 | - |
pyruvate | pH 7, 37°C | Methanosarcina barkeri | |
1.2.7.1 | 0.07 | - |
Oxidized benzyl viologen | clostridial ferredoxin, pH 7, 37°C | Methanosarcina barkeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 130000 | - |
gel filtration | Methanosarcina barkeri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | Methanosarcina barkeri | the enzyme is involved in catabolism of pyruvate | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | Methanosarcina barkeri DSM 804 | the enzyme is involved in catabolism of pyruvate | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.1 | Methanosarcina barkeri | P80521 and P80522 and P80523 and P80524 | P80521: subunit PorA, P80522: subunit PorB, P80523: subunit PorC, P80524: subunit PorD | - |
1.2.7.1 | Methanosarcina barkeri DSM 804 | P80521 and P80522 and P80523 and P80524 | P80521: subunit PorA, P80522: subunit PorB, P80523: subunit PorC, P80524: subunit PorD | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.1 | the enzyme is extremely oxygen sensitive, losing 90% of its activity upon exposure to air for 1 h at 0°C | Methanosarcina barkeri |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.1 | - |
Methanosarcina barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen | - |
Methanosarcina barkeri | propanoyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen | - |
Methanosarcina barkeri DSM 804 | propanoyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
1.2.7.1 | additional information | no oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, 3-hydroxypyruvate and oxaloacetate | Methanosarcina barkeri | ? | - |
? | |
1.2.7.1 | additional information | no oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, 3-hydroxypyruvate and oxaloacetate | Methanosarcina barkeri DSM 804 | ? | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | the enzyme is involved in catabolism of pyruvate | Methanosarcina barkeri | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | the deazaflavin, coenzyme F420, which has been proposed to be the physiological electron acceptor of pyruvate oxidoreductase in methanogens, is not reduced by the purified enzyme. In addition to ferredoxin and viologen dyes, flavin nucleotides serve as electron acceptors | Methanosarcina barkeri | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | the enzyme is involved in catabolism of pyruvate | Methanosarcina barkeri DSM 804 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + 2 oxidized ferredoxin | the deazaflavin, coenzyme F420, which has been proposed to be the physiological electron acceptor of pyruvate oxidoreductase in methanogens, is not reduced by the purified enzyme. In addition to ferredoxin and viologen dyes, flavin nucleotides serve as electron acceptors | Methanosarcina barkeri DSM 804 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.1 | ? | alphabetagammadelta structure, 1 * 48000 (subunit PorA) + 1 * 30000 (subunit PorB) + 1 * 25000 (subunit PorC) + 1 * 15000 (subunit PorD), SDS-PAGE | Methanosarcina barkeri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.1 | pyruvate:ferredoxin oxidoreductase | - |
Methanosarcina barkeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 37 | - |
assay at | Methanosarcina barkeri |
1.2.7.1 | 59 | - |
- |
Methanosarcina barkeri |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 40 | 70 | 40°C: about 50% of maximal activity, 70°C: about 45% of maximal activity | Methanosarcina barkeri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 7 | - |
- |
Methanosarcina barkeri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.1 | additional information | FAD, FMN and lipoic acid are not found | Methanosarcina barkeri | |
1.2.7.1 | thiamine diphosphate | the enzyme contained 1 mol/mol thiamine diphosphate | Methanosarcina barkeri |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.1 | 10 | - |
Sodium nitrite | pH 7, 37°C | Methanosarcina barkeri |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.7.1 | metabolism | the enzyme is involved in catabolism of pyruvate | Methanosarcina barkeri |