EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.B11 | recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)pLysS | Entamoeba histolytica |
6.2.1.13 | expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Entamoeba histolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.B11 | acetyl phosphate | - |
Entamoeba histolytica | |
6.2.1.B11 | AMP | slight inhibition | Entamoeba histolytica | |
6.2.1.B11 | ATP | ATP inhibits the acetate-forming direction of the reaction, with 50% inhibitory concentration of 0.81 mM, which is in the range of its physiological concentration. Diphosphate displays mixed inhibition versus each of the three substrates, acetyl-CoA, ADP, and phosphate. No inhibition by lactate and pyruvate | Entamoeba histolytica | |
6.2.1.B11 | cAMP | - |
Entamoeba histolytica | |
6.2.1.B11 | D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica | |
6.2.1.B11 | D-fructose 6-phosphate | - |
Entamoeba histolytica | |
6.2.1.B11 | D-glucose 6-phosphate | - |
Entamoeba histolytica | |
6.2.1.B11 | diphosphate | diphosphate inhibits the acetate-forming direction of the reaction, with 50% inhibitory concentration of 0.75 mM, which is in the range of its physiological concentration. ATP displays mixed inhibition versus each of the three substrates, acetyl-CoA, ADP, and phosphate | Entamoeba histolytica | |
6.2.1.B11 | glyoxylate | inhibits the activity in the acetate-forming direction | Entamoeba histolytica | |
6.2.1.B11 | NAD+ | - |
Entamoeba histolytica | |
6.2.1.B11 | NADH | - |
Entamoeba histolytica | |
6.2.1.13 | ATP | inhibits the acetate-forming direction of the reaction | Entamoeba histolytica | |
6.2.1.13 | Ca2+ | about 3% residual activity at 20 mM | Entamoeba histolytica | |
6.2.1.13 | Cu2+ | about 15% residual activity at 20 mM | Entamoeba histolytica | |
6.2.1.13 | D-glucose 6-phosphate | - |
Entamoeba histolytica | |
6.2.1.13 | diphosphate | inhibits the acetate-forming direction of the reaction (64% residual activity at 10 mM) | Entamoeba histolytica | |
6.2.1.13 | glyoxylate | 32% residual activity at 10 mM | Entamoeba histolytica | |
6.2.1.13 | NAD+ | 47% residual activity at 10 mM | Entamoeba histolytica | |
6.2.1.13 | Ni2+ | about 3% residual activity at 20 mM | Entamoeba histolytica | |
6.2.1.13 | Zn2+ | about 5% residual activity at 20 mM | Entamoeba histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.B11 | additional information | - |
additional information | Michaelis-Menten steady-state kinetic analysis, pseudo-first-order reaction kinetic determinations are performed in both directions of the reaction | Entamoeba histolytica | |
6.2.1.B11 | 0.032 | - |
propionyl-CoA | pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 0.04 | - |
acetyl-CoA | pH 7.3, 37°C, acetate-forming direction | Entamoeba histolytica | |
6.2.1.B11 | 0.2 | - |
CoA | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 0.71 | - |
ADP | pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 1.5 | - |
phosphate | pH 7.3, 37°C, propionate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 1.6 | - |
ADP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 1.6 | - |
CoA | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 1.8 | - |
phosphate | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 1.9 | - |
GDP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 7.2 | - |
ATP | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 10 | - |
GTP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 12 | - |
ATP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 14 | - |
acetate | pH 7.3, 37°C, acetyl-CoA-forming direction | Entamoeba histolytica | |
6.2.1.B11 | 29 | - |
propionate | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.13 | 0.032 | - |
propionyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 0.04 | - |
acetyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 0.2 | - |
CoA | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 0.71 | - |
ADP | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1.5 | - |
phosphate | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1.6 | - |
ADP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1.6 | - |
CoA | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1.8 | - |
phosphate | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1.9 | - |
GDP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 7.2 | - |
ATP | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 10 | - |
GTP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 12 | - |
ATP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 14 | - |
acetate | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 29 | - |
propionate | at pH 7.3 and 37°C | Entamoeba histolytica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.B11 | Ca2+ | can slightly substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.B11 | Co2+ | can partially substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.B11 | Cu2+ | can slightly substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.B11 | Mg2+ | required for activity, best divalent cation | Entamoeba histolytica | |
6.2.1.B11 | Mn2+ | can substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.B11 | Ni2+ | can slightly substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.B11 | Zn2+ | can slightly substitute for Mg2+ | Entamoeba histolytica | |
6.2.1.13 | Co2+ | 90% activity in the presence of 20 mM | Entamoeba histolytica | |
6.2.1.13 | Mg2+ | 100% activity in the presence of 20 mM | Entamoeba histolytica | |
6.2.1.13 | Mn2+ | 100% activity in the presence of 20 mM | Entamoeba histolytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.B11 | 150000 | - |
recombinant His6-tagged enzyme, gel filtration | Entamoeba histolytica |
6.2.1.13 | 150000 | - |
gel filtration | Entamoeba histolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.B11 | ATP + acetate + CoA | Entamoeba histolytica | - |
ADP + phosphate + acetyl-CoA | - |
r | |
6.2.1.B11 | GTP + acetate + CoA | Entamoeba histolytica | - |
GDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | ADP + phosphate + acetyl-CoA | Entamoeba histolytica | the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction | ATP + acetate + CoA | - |
r | |
6.2.1.13 | ATP + acetate + CoA | Entamoeba histolytica | preferred direction of reaction | ADP + phosphate + acetyl-CoA | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.B11 | Entamoeba histolytica | Q9NAT4 | - |
- |
6.2.1.13 | Entamoeba histolytica | C4LUV9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.B11 | recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2(DE3)pLysS by nickel affinity chromatography | Entamoeba histolytica |
6.2.1.13 | HisTrap HP nickel affinity column chromatography, and Superdex 200 gel filtration | Entamoeba histolytica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.B11 | trophozoite | - |
Entamoeba histolytica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.B11 | ADP + phosphate + acetyl-CoA | - |
Entamoeba histolytica | ATP + acetate + CoA | - |
r | |
6.2.1.B11 | ADP + phosphate + propionyl-CoA | - |
Entamoeba histolytica | ATP + propionate + CoA | - |
r | |
6.2.1.B11 | ATP + acetate + CoA | - |
Entamoeba histolytica | ADP + phosphate + acetyl-CoA | - |
r | |
6.2.1.B11 | ATP + propionate + CoA | - |
Entamoeba histolytica | ADP + phosphate + propionyl-CoA | - |
r | |
6.2.1.B11 | GDP + phosphate + acetyl-CoA | - |
Entamoeba histolytica | GTP + acetate + CoA | - |
r | |
6.2.1.B11 | GTP + acetate + CoA | - |
Entamoeba histolytica | GDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.B11 | GTP + acetate + CoA | GTP gives 57% of the activity with ATP | Entamoeba histolytica | GDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.B11 | ITP + acetate + CoA | ITP gives 15% of the activity with ATP | Entamoeba histolytica | IDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.B11 | additional information | the enzyme can utilize both ADP/ATP and GDP/GTP in the respective directions of the reaction. Less than 4% of maximal activity is observed with isobutyrate, valerate, isovalerate, hexanoate, heptanoate, octanoate, succinate, or phenylacetate as the acyl substrate. Activity observed with ATP (representing 100% activity) is nearly double that with GTP (57%) and substantially higher than the activities observed with ITP (15%), CTP (4.1%), or UTP (1.6%), no activity is observed with TTP or diphosphate. Enzymatic activity in the acetate-forming direction is determined by measuring the release of CoA with a sulfhydryl group (CoASH) from acyl-CoA by using Ellman's thiol reagent (DTNB). Production of NTB2- by CoASH cleavage of DTNB is measured spectrophotometrically at 412 nm. Activity in the acetate-forming direction is confirmed using the hexokinase/glucose-6-phosphate dehydrogenase-coupled assay. This assay couples ATP formation to the reduction of NADP+ to NADPH, which is measured spectrophotometrically at 340 nm | Entamoeba histolytica | ? | - |
? | |
6.2.1.13 | ADP + phosphate + acetyl-CoA | 100% activity | Entamoeba histolytica | ATP + acetate + CoA | - |
r | |
6.2.1.13 | ADP + phosphate + acetyl-CoA | the specific activity in the acetate-forming direction is approximately half that observed in the acetyl-CoA-forming direction | Entamoeba histolytica | ATP + acetate + CoA | - |
r | |
6.2.1.13 | ADP + phosphate + isobutyryl-CoA | less than 1% activity compared to acetyl-CoA | Entamoeba histolytica | ATP + isobutyrate + CoA | - |
r | |
6.2.1.13 | ADP + phosphate + isovaleryl-CoA | less than 1% activity compared to acetyl-CoA | Entamoeba histolytica | ATP + isovalerate + CoA | - |
r | |
6.2.1.13 | ADP + phosphate + propionyl-CoA | 16% activity compared to acetyl-CoA | Entamoeba histolytica | ATP + propionate + CoA | - |
r | |
6.2.1.13 | ATP + acetate + CoA | 100% activity | Entamoeba histolytica | ADP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | ATP + acetate + CoA | preferred direction of reaction | Entamoeba histolytica | ADP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | ATP + butyrate + CoA | 5.6% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + butyryl-CoA | - |
r | |
6.2.1.13 | ATP + heptanoate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + heptanoyl-CoA | - |
r | |
6.2.1.13 | ATP + hexanoate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + hexanoyl-CoA | - |
r | |
6.2.1.13 | ATP + isobutyrate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + isobutyryl-CoA | - |
r | |
6.2.1.13 | ATP + isovalerate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + isovaleryl-CoA | - |
r | |
6.2.1.13 | ATP + octanoate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + octanoyl-CoA | - |
r | |
6.2.1.13 | ATP + phenylacetate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + phenylacetyl-CoA | - |
r | |
6.2.1.13 | ATP + propionate + CoA | 72% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + propionyl-CoA | - |
r | |
6.2.1.13 | ATP + succinate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + succinyl-CoA | - |
r | |
6.2.1.13 | ATP + valerate + CoA | less than 4% activity compared to acetate | Entamoeba histolytica | ADP + phosphate + valeryl-CoA | - |
r | |
6.2.1.13 | CDP + phosphate + acetyl-CoA | 7% activity compared to ADP | Entamoeba histolytica | CTP + acetate + CoA | - |
r | |
6.2.1.13 | CTP + acetate + CoA | 4.1% activity compared to ATP | Entamoeba histolytica | CDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | GDP + phosphate + acetyl-CoA | 106% activity compared to ADP | Entamoeba histolytica | GTP + acetate + CoA | - |
r | |
6.2.1.13 | GTP + acetate + CoA | 57% activity compared to ATP | Entamoeba histolytica | GDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | IDP + phosphate + acetyl-CoA | 28% activity compared to ADP | Entamoeba histolytica | ITP + acetate + CoA | - |
r | |
6.2.1.13 | ITP + acetate + CoA | 15% activity compared to ATP | Entamoeba histolytica | IDP + phosphate + acetyl-CoA | - |
r | |
6.2.1.13 | additional information | no activity with diphosphate, AMP, TDP, TTP, butyryl-CoA, succinyl-CoA, or phenylacetyl-CoA | Entamoeba histolytica | ? | - |
? | |
6.2.1.13 | UDP + phosphate + acetyl-CoA | 2.8% activity compared to ADP | Entamoeba histolytica | UTP + acetate + CoA | - |
r | |
6.2.1.13 | UTP + acetate + CoA | 1.6% activity compared to ATP | Entamoeba histolytica | UDP + phosphate + acetyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.B11 | homodimer | 2 * 78999, recombinant His6-tagged enzyme, sequence calculation and SDS-PAGE | Entamoeba histolytica |
6.2.1.13 | homodimer | 2 * 78999, calculated from amino acid sequence | Entamoeba histolytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.B11 | ACD | - |
Entamoeba histolytica |
6.2.1.B11 | Acetyl-CoA synthetase (ADP-forming) | - |
Entamoeba histolytica |
6.2.1.B11 | ADP-forming acetyl coenzyme A synthetase | - |
Entamoeba histolytica |
6.2.1.B11 | EhACD | - |
Entamoeba histolytica |
6.2.1.B11 | More | cf. EC 6.2.1.13 | Entamoeba histolytica |
6.2.1.13 | ACD | - |
Entamoeba histolytica |
6.2.1.13 | acetate:CoA ligase [ADP-forming] | - |
Entamoeba histolytica |
6.2.1.13 | ADP-forming acetyl-coenzyme A synthetase | - |
Entamoeba histolytica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.B11 | 37 | - |
acetate-forming assay at | Entamoeba histolytica |
6.2.1.B11 | 55 | - |
acetyl-CoA-forming direction | Entamoeba histolytica |
6.2.1.13 | 55 | - |
- |
Entamoeba histolytica |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 37 | - |
the enzyme exhibits 70% activity at 37°C | Entamoeba histolytica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.B11 | 18 | - |
phosphate | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 21 | - |
propionyl-CoA | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 24 | - |
ADP | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 110 | - |
acetyl-CoA | pH 7.3, 37°C, acetate-forming direction | Entamoeba histolytica | |
6.2.1.B11 | 120 | - |
phosphate | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 140 | - |
ADP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 170 | - |
GDP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 180 | - |
GTP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 190 | - |
propionate | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 220 | - |
CoA | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 240 | - |
acetate | pH 7.3, 37°C, acetyl-CoA-forming direction | Entamoeba histolytica | |
6.2.1.B11 | 240 | - |
ATP | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 260 | - |
CoA | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 320 | - |
ATP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.13 | 18 | - |
phosphate | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 21 | - |
propionyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 24 | - |
ADP | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 110 | - |
acetyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 120 | - |
phosphate | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 140 | - |
ADP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 170 | - |
GDP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 180 | - |
GTP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 190 | - |
propionate | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 220 | - |
CoA | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 240 | - |
acetate | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 240 | - |
ATP | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 260 | - |
CoA | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 320 | - |
ATP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.B11 | 7.3 | - |
assay at | Entamoeba histolytica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.B11 | ADP | - |
Entamoeba histolytica | |
6.2.1.B11 | ATP | - |
Entamoeba histolytica | |
6.2.1.B11 | GDP | - |
Entamoeba histolytica | |
6.2.1.B11 | GTP | - |
Entamoeba histolytica | |
6.2.1.13 | ADP | - |
Entamoeba histolytica | |
6.2.1.13 | ATP | - |
Entamoeba histolytica | |
6.2.1.13 | GDP | - |
Entamoeba histolytica | |
6.2.1.13 | GTP | - |
Entamoeba histolytica |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
6.2.1.B11 | 0.81 | - |
pH 7.3, 37°C, acetate-forming direction | Entamoeba histolytica | ATP | |
6.2.1.B11 | 0.83 | - |
pH 7.3, 37°C, acetate-forming direction | Entamoeba histolytica | diphosphate | |
6.2.1.13 | 0.81 | - |
at pH 7.3 and 37°C | Entamoeba histolytica | ATP | |
6.2.1.13 | 0.83 | - |
at pH 7.3 and 37°C | Entamoeba histolytica | diphosphate | |
6.2.1.13 | 10 | - |
at pH 7.3 and 37°C | Entamoeba histolytica | D-glucose 6-phosphate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.2.1.B11 | physiological function | enzyme ACD is regulated through inhibition by ATP and diphosphate in the acetate-forming direction | Entamoeba histolytica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.B11 | 6.3 | - |
propionate | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 12 | - |
phosphate | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 16 | - |
acetate | pH 7.3, 37°C, acetyl-CoA-forming direction | Entamoeba histolytica | |
6.2.1.B11 | 18 | - |
GTP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 27 | - |
ATP | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 33 | - |
ATP | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 34 | - |
ADP | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 65 | - |
phosphate | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 89 | - |
ADP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 90 | - |
GDP | pH 7.3, 37°C, acetate-forming direction, with acetyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 160 | - |
CoA | pH 7.3, 37°C, propionyl-CoA-forming direction, with propionate | Entamoeba histolytica | |
6.2.1.B11 | 650 | - |
propionyl-CoA | pH 7.3, 37°C, acetate-forming direction, with propionyl-CoA | Entamoeba histolytica | |
6.2.1.B11 | 1100 | - |
CoA | pH 7.3, 37°C, acetyl-CoA-forming direction, with acetate | Entamoeba histolytica | |
6.2.1.B11 | 2600 | - |
acetyl-CoA | pH 7.3, 37°C, acetate-forming direction | Entamoeba histolytica | |
6.2.1.13 | 6.3 | - |
propionate | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 12 | - |
phosphate | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 16 | - |
acetate | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 18 | - |
GTP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 27 | - |
ATP | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 33 | - |
ATP | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 34 | - |
ADP | with propionyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 65 | - |
phosphate | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 89 | - |
ADP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 90 | - |
GDP | with acetyl-CoA as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 160 | - |
CoA | with propionate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 650 | - |
propionyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 1100 | - |
CoA | with acetate as cosubstrate, at pH 7.3 and 37°C | Entamoeba histolytica | |
6.2.1.13 | 2600 | - |
acetyl-CoA | at pH 7.3 and 37°C | Entamoeba histolytica |