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Literature summary extracted from

  • McNeill, L.A.; Brown, T.J.N.; Sami, M.; Clifton, I.J.; Burzlaff, N.I.; Claridge, T.D.W.; Adlington, R.M.; Baldwin, J.E.; Rutledge, P.J.; Schofield, C.J.
    Terminally truncated isopenicillin N synthase generates a dithioester product evidence for a thioaldehyde intermediate during catalysis and a new mode of reaction for non-heme iron oxidases (2017), Chemistry, 23, 12815-12824 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.1 K98E the mutant shows about 8% of wild type activity Aspergillus nidulans
1.21.3.1 L223I the mutant shows about 33% of wild type activity Aspergillus nidulans
1.21.3.1 L223V the mutant shows about 33% of wild type activity Aspergillus nidulans
1.21.3.1 L231I the mutant shows about 35% of wild type activity Aspergillus nidulans
1.21.3.1 L231T inactive Aspergillus nidulans
1.21.3.1 L231V the mutant shows about 25% of wild type activity Aspergillus nidulans
1.21.3.1 P283A the mutant shows about 12% of wild type activity Aspergillus nidulans
1.21.3.1 P283I the mutant shows about 10% of wild type activity Aspergillus nidulans
1.21.3.1 P283L inactive Aspergillus nidulans
1.21.3.1 P283V the mutant shows about 9% of wild type activity Aspergillus nidulans
1.21.3.1 V272T the mutant shows about 27% of wild type activity Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+ dependent on Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans
-
 isopenicillin N + H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans ATCC 38163
-
 isopenicillin N + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Aspergillus nidulans P05326
-
-
1.21.3.1 Aspergillus nidulans ATCC 38163 P05326
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.1 Sepharose S75 gel filtration and MonoQ resin column chromatography Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans (2S)-2-amino-6-([(2R)-1-[(1S)-1-carboxy-2-methylpropoxy]-1,3-dioxo-3-sulfanylpropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
1.21.3.1 delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ATCC 38163 (2S)-2-amino-6-([(2R)-1-[(1S)-1-carboxy-2-methylpropoxy]-1,3-dioxo-3-sulfanylpropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
1.21.3.1 delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ?
-
 ?
1.21.3.1 delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ATCC 38163 ?
-
 ?
1.21.3.1 L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans (2S)-2-amino-6-([(2S)-1-[(carboxymethyl)amino]-3,3-dihydroxy-1-oxopropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
1.21.3.1 L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ATCC 38163 (2S)-2-amino-6-([(2S)-1-[(carboxymethyl)amino]-3,3-dihydroxy-1-oxopropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
1.21.3.1 L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ?
-
 ?
1.21.3.1 L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ATCC 38163 ?
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans isopenicillin N + H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans ATCC 38163 isopenicillin N + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Aspergillus nidulans
1.21.3.1 isopenicillin N synthase
-
 Aspergillus nidulans