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Literature summary extracted from

  • Thomas, M.; Burkart, M.; Walsh, C.
    Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis (2002), Chem. Biol., 9, 171-184 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.14 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor
6.2.1.53 expression in Escherichia coli Pseudomonas fluorescens
6.2.1.53 expression in Escherichia coli Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.53 0.51
-
 L-proline pH 7.5, 25°C Pseudomonas fluorescens
6.2.1.53 1.54
-
 L-proline pH 7.5, 25°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.14 S-(L-prolyl)-[peptidyl-carrier protein] + electron-transfer flavoprotein Streptomyces coelicolor
-
 S-(1H-pyrrole-2-carbonyl)-[peptidyl-carrier protein] + reduced electron-transfer flavoprotein
-
 ?
1.3.8.14 S-(L-prolyl)-[peptidyl-carrier protein] + electron-transfer flavoprotein Streptomyces coelicolor A(3)2
-
 S-(1H-pyrrole-2-carbonyl)-[peptidyl-carrier protein] + reduced electron-transfer flavoprotein
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens
-
 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Streptomyces coelicolor
-
 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Streptomyces coelicolor A(3)2
-
 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens ATCC BAA-4770
-
 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline Pseudomonas fluorescens
-
 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline Streptomyces coelicolor
-
 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline Streptomyces coelicolor A(3)2
-
 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline Pseudomonas fluorescens ATCC BAA-4770
-
 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens
-
 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein] Streptomyces coelicolor
-
 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein] Streptomyces coelicolor A(3)2
-
 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens ATCC BAA-4770
-
 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 additional information Pseudomonas fluorescens very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
 ?
6.2.1.53 additional information Streptomyces coelicolor very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
 ?
6.2.1.53 additional information Streptomyces coelicolor A(3)2 very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
 ?
6.2.1.53 additional information Pseudomonas fluorescens ATCC BAA-4770 very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.14 Streptomyces coelicolor
-
-
-
1.3.8.14 Streptomyces coelicolor A(3)2
-
-
-
6.2.1.53 Pseudomonas fluorescens Q4KCY5
-
-
6.2.1.53 Pseudomonas fluorescens ATCC BAA-4770 Q4KCY5
-
-
6.2.1.53 Streptomyces coelicolor Q4KCY5
-
-
6.2.1.53 Streptomyces coelicolor A(3)2 Q4KCY5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.14 Q sepharose column chromatography Streptomyces coelicolor
6.2.1.53 recombinant protein Pseudomonas fluorescens
6.2.1.53 recombinant protein Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.14 additional information no activity with L-prolyl-S-CoA Streptomyces coelicolor ?
-
 ?
1.3.8.14 additional information no activity with L-prolyl-S-CoA Streptomyces coelicolor A(3)2 ?
-
 ?
1.3.8.14 S-(L-prolyl)-[peptidyl-carrier protein] + electron-transfer flavoprotein
-
 Streptomyces coelicolor S-(1H-pyrrole-2-carbonyl)-[peptidyl-carrier protein] + reduced electron-transfer flavoprotein
-
 ?
1.3.8.14 S-(L-prolyl)-[peptidyl-carrier protein] + electron-transfer flavoprotein
-
 Streptomyces coelicolor A(3)2 S-(1H-pyrrole-2-carbonyl)-[peptidyl-carrier protein] + reduced electron-transfer flavoprotein
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
 Pseudomonas fluorescens AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
 Streptomyces coelicolor AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
 Streptomyces coelicolor A(3)2 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 (L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
 Pseudomonas fluorescens ATCC BAA-4770 AMP + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline
-
 Pseudomonas fluorescens diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline
-
 Streptomyces coelicolor diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline
-
 Streptomyces coelicolor A(3)2 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline
-
 Pseudomonas fluorescens ATCC BAA-4770 diphosphate + (L-prolyl)adenylate
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
-
 Pseudomonas fluorescens AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
-
 Streptomyces coelicolor AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein]
-
 Pseudomonas fluorescens AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein]
-
 Streptomyces coelicolor AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein]
-
 Streptomyces coelicolor A(3)2 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 ATP + L-proline + holo-[L-prolyl-carrier protein]
-
 Pseudomonas fluorescens ATCC BAA-4770 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine Pseudomonas fluorescens ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine Streptomyces coelicolor ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Pseudomonas fluorescens ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Streptomyces coelicolor ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine Streptomyces coelicolor A(3)2 ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Streptomyces coelicolor A(3)2 ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine Pseudomonas fluorescens ATCC BAA-4770 ?
-
 ?
6.2.1.53 additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Pseudomonas fluorescens ATCC BAA-4770 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.14 ? x * 40000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.3.8.14 L-prolyl-S-PCP dehydrogenase
-
 Streptomyces coelicolor
1.3.8.14 pltE
-
 Streptomyces coelicolor
1.3.8.14 redW
-
 Streptomyces coelicolor
1.3.8.14 SC2E9.20
-
 Streptomyces coelicolor
6.2.1.53 L-prolyl-AMP ligase
-
 Pseudomonas fluorescens
6.2.1.53 L-prolyl-AMP ligase
-
 Streptomyces coelicolor
6.2.1.53 pltF
-
 Pseudomonas fluorescens
6.2.1.53 SC3F7.11
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.53 25
-
 assay at Pseudomonas fluorescens
6.2.1.53 25
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.53 2.8
-
 L-proline pH 7.5, 25°C Streptomyces coelicolor
6.2.1.53 5.5
-
 L-proline pH 7.5, 25°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.53 7.5
-
 assay at Pseudomonas fluorescens
6.2.1.53 7.5
-
 assay at Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.14 FAD
-
 Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
6.2.1.53 physiological function involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein Pseudomonas fluorescens
6.2.1.53 physiological function involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein Streptomyces coelicolor