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Literature summary extracted from

  • Gusakov, A.; Bulakhov, A.; Demin, I.; Sinitsyn, A.
    Monitoring of reactions catalyzed by lytic polysaccharide monooxygenases using highly-sensitive fluorimetric assay of the oxygen consumption rate (2017), Carbohydr. Res., 452, 156-161 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.54 analysis feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction Thermothelomyces thermophilus
1.14.99.54 analysis feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction Thermothielavioides terrestris
1.14.99.54 analysis feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.54 EDTA 2 mM, complete loss of activity Thermothielavioides terrestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.54 Cu2+ EDTA-inhibited enzyme can be reactivated by 20 mM Cu2+ within 1 h Thermothielavioides terrestris

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Thermothelomyces thermophilus A0A0H4K9X4
-
-
1.14.99.54 Thermothielavioides terrestris D0VWZ9
-
-
1.14.99.54 Trichoderma reesei O14405
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 cellulose + ascorbic acid + O2
-
Thermothelomyces thermophilus ? + dehydroascorbate + H2O
-
?
1.14.99.54 cellulose + ascorbic acid + O2
-
Thermothielavioides terrestris ? + dehydroascorbate + H2O
-
?
1.14.99.54 cellulose + ascorbic acid + O2
-
Trichoderma reesei ? + dehydroascorbate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 Cel61a
-
Trichoderma reesei
1.14.99.54 gh61e
-
Thermothielavioides terrestris
1.14.99.54 LPMO9A
-
Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.54 0.015
-
cellulose pH 5.0, 37°C Thermothielavioides terrestris
1.14.99.54 0.021
-
cellulose pH 5.0, 37°C Thermothelomyces thermophilus
1.14.99.54 0.021
-
cellulose pH 5.0, 37°C Trichoderma reesei