EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.99.54 | analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothelomyces thermophilus |
1.14.99.54 | analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothielavioides terrestris |
1.14.99.54 | analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Trichoderma reesei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.54 | EDTA | 2 mM, complete loss of activity | Thermothielavioides terrestris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.54 | Cu2+ | EDTA-inhibited enzyme can be reactivated by 20 mM Cu2+ within 1 h | Thermothielavioides terrestris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.54 | Thermothelomyces thermophilus | A0A0H4K9X4 | - |
- |
1.14.99.54 | Thermothielavioides terrestris | D0VWZ9 | - |
- |
1.14.99.54 | Trichoderma reesei | O14405 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.54 | cellulose + ascorbic acid + O2 | - |
Thermothelomyces thermophilus | ? + dehydroascorbate + H2O | - |
? | |
1.14.99.54 | cellulose + ascorbic acid + O2 | - |
Thermothielavioides terrestris | ? + dehydroascorbate + H2O | - |
? | |
1.14.99.54 | cellulose + ascorbic acid + O2 | - |
Trichoderma reesei | ? + dehydroascorbate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.54 | Cel61a | - |
Trichoderma reesei |
1.14.99.54 | gh61e | - |
Thermothielavioides terrestris |
1.14.99.54 | LPMO9A | - |
Thermothelomyces thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.99.54 | 0.015 | - |
cellulose | pH 5.0, 37°C | Thermothielavioides terrestris | |
1.14.99.54 | 0.021 | - |
cellulose | pH 5.0, 37°C | Thermothelomyces thermophilus | |
1.14.99.54 | 0.021 | - |
cellulose | pH 5.0, 37°C | Trichoderma reesei |