BRENDA - Enzyme Database

CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis

Mao, H.; Shen, Q.; Wang, Q.; Biotechnol. Lett. 39, 1709-1716 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.86
gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Zea mays
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.86
additional information
enzyme modification by N-terminal deletion of the first 43 or 40 amino acids and replacing with 10 amino acid lysine-rich sequences of MAKKTSSKGK
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.86
chloroplast
-
Zea mays
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Zea mays
-
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
additional information
Zea mays
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.86
Zea mays
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.86
additional information
CYP701A26 shows constitutive expression in active growing tissues and no inducible expression
Zea mays
-
1.14.14.86
seedling
-
Zea mays
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.86
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
744583
Zea mays
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744583
Zea mays
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
additional information
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
744583
Zea mays
?
-
-
-
-
1.14.14.86
additional information
CYP701A26 exhibits substrate promiscuity to catalyze oxidation of other labdane related diterpenes
744583
Zea mays
?
-
-
-
-
1.14.14.86
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.86
cytochrome P450
-
Zea mays
1.14.14.86
NADPH
-
Zea mays
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.86
gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
Zea mays
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.86
cytochrome P450
-
Zea mays
1.14.14.86
NADPH
-
Zea mays
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.86
additional information
enzyme modification by N-terminal deletion of the first 43 or 40 amino acids and replacing with 10 amino acid lysine-rich sequences of MAKKTSSKGK
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.86
chloroplast
-
Zea mays
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Zea mays
-
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
additional information
Zea mays
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.86
additional information
CYP701A26 shows constitutive expression in active growing tissues and no inducible expression
Zea mays
-
1.14.14.86
seedling
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.86
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
744583
Zea mays
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
744583
Zea mays
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
additional information
CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
744583
Zea mays
?
-
-
-
-
1.14.14.86
additional information
CYP701A26 exhibits substrate promiscuity to catalyze oxidation of other labdane related diterpenes
744583
Zea mays
?
-
-
-
-
1.14.14.86
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
744583
Zea mays
3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.14.86
Zea mays
constitutive expression in active growing tissues and no inducible expression; CYP701A26 shows no inducible expression
additional information
General Information
EC Number
General Information
Commentary
Organism
1.14.14.86
evolution
the enzyme belong to the CYP701A subfamily
Zea mays
1.14.14.86
metabolism
oxidation of ent-kaurene to ent-kaurenoic acid is involved in gibberellin biosynthesis
Zea mays
1.14.14.86
physiological function
the enzyme is involved in gibberellin biosynthesis and catalyzes carboxylation on C19 of ent-kaurene to generate ent-kaurenoic acid in plastids. The tetracyclic diterpenoid plant hormones, gibberellins, play important roles during the entire life cycle of higher plants including cell elongation, flowering and fruit set, seed germination, and many other developmental processes
Zea mays
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.86
evolution
the enzyme belong to the CYP701A subfamily
Zea mays
1.14.14.86
metabolism
oxidation of ent-kaurene to ent-kaurenoic acid is involved in gibberellin biosynthesis
Zea mays
1.14.14.86
physiological function
the enzyme is involved in gibberellin biosynthesis and catalyzes carboxylation on C19 of ent-kaurene to generate ent-kaurenoic acid in plastids. The tetracyclic diterpenoid plant hormones, gibberellins, play important roles during the entire life cycle of higher plants including cell elongation, flowering and fruit set, seed germination, and many other developmental processes
Zea mays
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.14.86
Zea mays
constitutive expression in active growing tissues and no inducible expression; CYP701A26 shows no inducible expression
additional information