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Literature summary extracted from

  • Mao, H.; Shen, Q.; Wang, Q.
    CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis (2017), Biotechnol. Lett., 39, 1709-1716 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.86 gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.86 additional information enzyme modification by N-terminal deletion of the first 43 or 40 amino acids and replacing with 10 amino acid lysine-rich sequences of MAKKTSSKGK Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.86 chloroplast
-
Zea mays 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Zea mays
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Zea mays
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Zea mays
-
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Zea mays overall reaction ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Zea mays
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 additional information Zea mays CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.86 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.86 additional information CYP701A26 shows constitutive expression in active growing tissues and no inducible expression Zea mays
-
1.14.14.86 seedling
-
Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.86 ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
Zea mays ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Zea mays ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.86 additional information CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid Zea mays ?
-
?
1.14.14.86 additional information CYP701A26 exhibits substrate promiscuity to catalyze oxidation of other labdane related diterpenes Zea mays ?
-
?
1.14.14.86 syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays 3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.86 CYP701A26
-
Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.86 cytochrome P450
-
Zea mays
1.14.14.86 NADPH
-
Zea mays

Expression

EC Number Organism Comment Expression
1.14.14.86 Zea mays constitutive expression in active growing tissues and no inducible expression additional information
1.14.14.86 Zea mays CYP701A26 shows no inducible expression additional information

General Information

EC Number General Information Comment Organism
1.14.14.86 evolution the enzyme belong to the CYP701A subfamily Zea mays
1.14.14.86 metabolism oxidation of ent-kaurene to ent-kaurenoic acid is involved in gibberellin biosynthesis Zea mays
1.14.14.86 physiological function the enzyme is involved in gibberellin biosynthesis and catalyzes carboxylation on C19 of ent-kaurene to generate ent-kaurenoic acid in plastids. The tetracyclic diterpenoid plant hormones, gibberellins, play important roles during the entire life cycle of higher plants including cell elongation, flowering and fruit set, seed germination, and many other developmental processes Zea mays