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Literature summary extracted from

  • Zheng, M.; Huang, Y.; Zheng, L.; Sun, Z.; Wang, L.
    Cloning, expression and stress-respondent transcription of long-chain acyl-coenzyme A synthetase cDNA gene of Nannochloropsis gaditana and its involvement in the biosynthesis of eicosapentaenoic and decosahexaenoic acids (2014), Biotechnol. Lett., 36, 141-145 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.3 expressed in Saccharomyces cerevisiae Nannochloropsis gaditana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.3 ATP + a long-chain fatty acid + CoA Nannochloropsis gaditana the enzyme can elongate and desaturate fatty acids up to 22:6 AMP + diphosphate + a long-chain acyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Nannochloropsis gaditana S5PTC7
-
-
6.2.1.3 Nannochloropsis gaditana HH-1 S5PTC7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + a long-chain fatty acid + CoA the enzyme can elongate and desaturate fatty acids up to 22:6 Nannochloropsis gaditana AMP + diphosphate + a long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + arachidonate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + arachidonoyl-CoA
-
 ?
6.2.1.3 ATP + arachidonate + CoA
-
 Nannochloropsis gaditana HH-1 AMP + diphosphate + arachidonoyl-CoA
-
 ?
6.2.1.3 ATP + dihomo-gamma-linolenate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + dihomo-gamma-linolenoyl-CoA
-
 ?
6.2.1.3 ATP + docosahexaenoate + CoA second best substrate Nannochloropsis gaditana AMP + diphosphate + docosahexaenoyl-CoA
-
 ?
6.2.1.3 ATP + eicosadienoate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + eicosadienoyl-CoA
-
 ?
6.2.1.3 ATP + eicosapentaenoate + CoA best substrate Nannochloropsis gaditana AMP + diphosphate + eicosapentaenoyl-CoA
-
 ?
6.2.1.3 ATP + eicosapentaenoate + CoA best substrate Nannochloropsis gaditana HH-1 AMP + diphosphate + eicosapentaenoyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.3 ATP + oleate + CoA
-
 Nannochloropsis gaditana HH-1 AMP + diphosphate + oleoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 ATP + palmitate + CoA
-
 Nannochloropsis gaditana HH-1 AMP + diphosphate + palmitoyl-CoA
-
 ?
6.2.1.3 ATP + palmitoleate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + palmitoleoyl-CoA
-
 ?
6.2.1.3 ATP + stearate + CoA
-
 Nannochloropsis gaditana AMP + diphosphate + stearoyl-CoA
-
 ?
6.2.1.3 additional information low activity with caprate, laurate, myristate, eicosanoate, and docosanoate Nannochloropsis gaditana ?
-
 ?
6.2.1.3 additional information low activity with caprate, laurate, myristate, eicosanoate, and docosanoate Nannochloropsis gaditana HH-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 ? x * 71000, calculated from amino acid sequence Nannochloropsis gaditana

Synonyms

EC Number Synonyms Comment Organism
6.2.1.3 LACS
-
 Nannochloropsis gaditana
6.2.1.3 Long-chain acyl-coenzyme A synthetase
-
 Nannochloropsis gaditana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
 Nannochloropsis gaditana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.3 Nannochloropsis gaditana calculated from amino acid sequence
-
 7.8

Expression

EC Number Organism Comment Expression
6.2.1.3 Nannochloropsis gaditana enzyme transcription is up-regulated when treated by nitrogen and iron excess and at 15 and 35°C up