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Literature summary extracted from

  • Misal, S.A.; Lingojwar, D.P.; Lokhande, M.N.; Lokhande, P.D.; Gawai, K.R.
    Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus (2014), Biotechnol. Lett., 36, 127-131 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.17 0.016
-
NADH pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 0.034
-
Amaranth pH 7.4, 37°C Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.17 29000
-
gel filtration and PAGE Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.17 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.17
-
Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.17 18
-
substrate orange G, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 22
-
substrate amaranth, cofactor NADPH, pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 25
-
substrate amaranth, cofactor FADH2, pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 26
-
substrate amaranth, cofactor FMNH2, pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 63
-
substrate fast red E, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus
1.7.1.17 74
-
substrate amaranth, cofactor NADH, pH 7.4, 37°C Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.17 amaranth + 2 NADH + 2 H+
-
Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
?
1.7.1.17 amaranth + 2 NADPH + 2 H+
-
Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NADP+
-
?
1.7.1.17 fast red E + 2 NADH + 2 H+
-
Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NAD+
-
?
1.7.1.17 fast red E + 2 NADPH + 2 H+
-
Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NADP+
-
?
1.7.1.17 additional information enzyme additionally acts as nitroreductase and selectively reduces the nitro group of 2-nitrophenol, 4-nitrobenzoic acid, 2-nitro-benzaldehyde and 3-nitrophenol Lysinibacillus sphaericus ?
-
?
1.7.1.17 orange G + 2 NADH + 2 H+
-
Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NAD+
-
?
1.7.1.17 orange G + 2 NADPH + 2 H+
-
Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.7.1.17 monomer 1 * 29000, SDS-PAGE Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.17 75
-
-
Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.17 70
-
stable for 30 min Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.17 7.4
-
-
Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.17 FADH2
-
Lysinibacillus sphaericus
1.7.1.17 FMNH2
-
Lysinibacillus sphaericus
1.7.1.17 NADH NADH is the preferred cofactor Lysinibacillus sphaericus
1.7.1.17 NADPH
-
Lysinibacillus sphaericus