EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.17 | 0.016 | - |
NADH | pH 7.4, 37°C | Lysinibacillus sphaericus | |
1.7.1.17 | 0.034 | - |
Amaranth | pH 7.4, 37°C | Lysinibacillus sphaericus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.1.17 | 29000 | - |
gel filtration and PAGE | Lysinibacillus sphaericus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.17 | Lysinibacillus sphaericus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.17 | - |
Lysinibacillus sphaericus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.1.17 | 18 | - |
substrate orange G, cofactor NADH, pH 7.4, 37°C | Lysinibacillus sphaericus |
1.7.1.17 | 22 | - |
substrate amaranth, cofactor NADPH, pH 7.4, 37°C | Lysinibacillus sphaericus |
1.7.1.17 | 25 | - |
substrate amaranth, cofactor FADH2, pH 7.4, 37°C | Lysinibacillus sphaericus |
1.7.1.17 | 26 | - |
substrate amaranth, cofactor FMNH2, pH 7.4, 37°C | Lysinibacillus sphaericus |
1.7.1.17 | 63 | - |
substrate fast red E, cofactor NADH, pH 7.4, 37°C | Lysinibacillus sphaericus |
1.7.1.17 | 74 | - |
substrate amaranth, cofactor NADH, pH 7.4, 37°C | Lysinibacillus sphaericus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.17 | amaranth + 2 NADH + 2 H+ | - |
Lysinibacillus sphaericus | 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ | - |
? | |
1.7.1.17 | amaranth + 2 NADPH + 2 H+ | - |
Lysinibacillus sphaericus | 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NADP+ | - |
? | |
1.7.1.17 | fast red E + 2 NADH + 2 H+ | - |
Lysinibacillus sphaericus | 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NAD+ | - |
? | |
1.7.1.17 | fast red E + 2 NADPH + 2 H+ | - |
Lysinibacillus sphaericus | 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NADP+ | - |
? | |
1.7.1.17 | additional information | enzyme additionally acts as nitroreductase and selectively reduces the nitro group of 2-nitrophenol, 4-nitrobenzoic acid, 2-nitro-benzaldehyde and 3-nitrophenol | Lysinibacillus sphaericus | ? | - |
? | |
1.7.1.17 | orange G + 2 NADH + 2 H+ | - |
Lysinibacillus sphaericus | aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NAD+ | - |
? | |
1.7.1.17 | orange G + 2 NADPH + 2 H+ | - |
Lysinibacillus sphaericus | aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.1.17 | monomer | 1 * 29000, SDS-PAGE | Lysinibacillus sphaericus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.1.17 | 75 | - |
- |
Lysinibacillus sphaericus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.1.17 | 70 | - |
stable for 30 min | Lysinibacillus sphaericus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.1.17 | 7.4 | - |
- |
Lysinibacillus sphaericus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.17 | FADH2 | - |
Lysinibacillus sphaericus | |
1.7.1.17 | FMNH2 | - |
Lysinibacillus sphaericus | |
1.7.1.17 | NADH | NADH is the preferred cofactor | Lysinibacillus sphaericus | |
1.7.1.17 | NADPH | - |
Lysinibacillus sphaericus |