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Literature summary extracted from

  • Fraebel, S.; Krischke, M.; Staniek, A.; Warzecha, H.
    Recombinant flavin-dependent halogenases are functional in tobacco chloroplasts without co-expression of flavin reductase genes (2016), Biotechnol. J., 11, 1586-1594 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.9 gene rebH, functional recombinant expression of Yfp-tagged enzyme in Nicotianan benthamiana leaf cytosol via Agrobacterium tumefaciens tranfection method under control of the CaMV 35S promoter leading to formation of 7-chlorotryptophan, coexpression with gene rebF from Lechevalieria aerocolonigenes, different genetic constructs, overview. Coexpression and concerted activity of tryptophan 6-halogenase Stth, EC 1.14.19.59, and RebH lead to formation of 6,7-halotryptophan Lentzea aerocolonigenes
1.14.19.59 gene stth, functional recombinant expression of Yfp-tagged enzyme in Nicotianan benthamiana leaves via Agrobacterium tumefaciens strain EHA105 tranfection method under control of the CaMV 35S promoter leading to formation of 6-chlorotryptophan, coexpression with gene rebF from Lechevalieria aerocolonigenes, different genetic constructs, overview. Coexpression and concerted activity of Stth and tryptophan 7-halogenase RebH, EC 1.14.19.9, lead to formation of 6,7-halotryptophan Streptomyces toxytricini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.9 6-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ Lentzea aerocolonigenes
-
 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.9 L-tryptophan + FADH2 + chloride + O2 + H+ Lentzea aerocolonigenes
-
 7-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.59 7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ Streptomyces toxytricini
-
 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.59 L-tryptophan + FADH2 + chloride + O2 + H+ Streptomyces toxytricini
-
 6-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.9 Lentzea aerocolonigenes Q8KHZ8
-
-
1.14.19.59 Streptomyces toxytricini E9P162
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.9 6-chloro-L-tryptophan + FADH2 + chloride + O2 + H+
-
 Lentzea aerocolonigenes 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.9 L-tryptophan + FADH2 + chloride + O2 + H+
-
 Lentzea aerocolonigenes 7-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.59 7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+
-
 Streptomyces toxytricini 6,7-dichloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.59 L-tryptophan + FADH2 + chloride + O2 + H+
-
 Streptomyces toxytricini 6-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.9 RebH
-
 Lentzea aerocolonigenes
1.14.19.59 sttH
-
 Streptomyces toxytricini

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.9 FAD
-
 Lentzea aerocolonigenes
1.14.19.59 FAD
-
 Streptomyces toxytricini