EC Number | Application | Comment | Organism |
---|---|---|---|
1.11.1.14 | industry | the enzyme can be used as biocatalytic in delignification (which is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability) iIn the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives | Agaricus bisporus |
1.11.1.14 | industry | the enzyme can be used as biocatalytic in delignification (which is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability) iIn the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives | Phanerodontia chrysosporium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | additional information | - |
additional information | comparison of kinetic parameters of tyrosinase (EC 1.14.18.1) and lignin peroxidase (EC 1.11.1.14) (LiP) using veratryl alcohol as the substrate, overview | Agaricus bisporus | |
1.11.1.14 | additional information | - |
additional information | comparison of kinetic parameters of tyrosinase (EC 1.14.18.1) and lignin peroxidase (EC 1.11.1.14) (LiP) using veratryl alcohol as the substrate, overview | Phanerodontia chrysosporium | |
1.11.1.14 | 0.31 | - |
veratryl alcohol | pH 6.5, 30°C | Agaricus bisporus | |
1.11.1.14 | 3.54 | - |
veratryl alcohol | pH 6.5, 30°C | Phanerodontia chrysosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.14 | additional information | Agaricus bisporus | tyrosinase (EC 1.14.18.1) has a promiscuous activity for oxidizing the lignin-related nonphenolic substrate veratryl alcohol, catalyzing the reaction of heme-containing lignin peroxidase, LiP, EC 1.11.1.14. Tyrosinase exhibits a broad substrate specificity for various phenolic compounds | ? | - |
? | |
1.11.1.14 | veratryl alcohol + H2O2 | Agaricus bisporus | - |
veratraldehyde + H2O | - |
? | |
1.11.1.14 | veratryl alcohol + H2O2 | Phanerodontia chrysosporium | - |
veratraldehyde + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.14 | Agaricus bisporus | - |
- |
- |
1.11.1.14 | Phanerodontia chrysosporium | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.11.1.14 | commercial preparation | - |
Agaricus bisporus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 0.0525 | - |
substrate veratryl alcohol, pH 6.5, 30°C | Agaricus bisporus |
1.11.1.14 | 0.77 | - |
substrate 4-phenoxyphenol, pH 6.5, 30°C | Agaricus bisporus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.14 | 4-phenoxyphenol + H2O2 | cleavage of 4-O-5 bond in 4-phenoxyphenol by the catalytic promiscuity of tyrosinase | Agaricus bisporus | phenol + 1,4-benzoquinone | - |
? | |
1.11.1.14 | guaiacyl glycerol-beta-guaiacyl ether + H2O2 | a dimeric lignin model compound, derivatization with tetramethylsilane is carried out to analyze guaiacyl glycerol-beta-guaiacyl ether, GGE. The catalytic promiscuity of tyrosinase seemed to cleave the Calpha-Cbeta bond in GGE, yielding vanillin and possibly an unstable o-(2-hydroxyethyl)guaiacol radical. The unstable o-(2-hydroxyethyl)guaiacol radical might be further catalyzed to guaiacol and 2-hydroxyacetaldehyde by the tyrosinase, and then guaiacol might be polymerized to an unidentified product | Agaricus bisporus | vanillin + guaiacol + 2-hydroxyacetaldehyde + H2O | - |
? | |
1.11.1.14 | additional information | tyrosinase (EC 1.14.18.1) has a promiscuous activity for oxidizing the lignin-related nonphenolic substrate veratryl alcohol, catalyzing the reaction of heme-containing lignin peroxidase, LiP, EC 1.11.1.14. Tyrosinase exhibits a broad substrate specificity for various phenolic compounds | Agaricus bisporus | ? | - |
? | |
1.11.1.14 | veratryl alcohol + H2O2 | - |
Agaricus bisporus | veratraldehyde + H2O | - |
? | |
1.11.1.14 | veratryl alcohol + H2O2 | - |
Phanerodontia chrysosporium | veratraldehyde + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.14 | heme-containing lignin peroxidase | - |
Phanerodontia chrysosporium |
1.11.1.14 | heme-containing peroxidase | - |
Phanerodontia chrysosporium |
1.11.1.14 | LIP | - |
Phanerodontia chrysosporium |
1.11.1.14 | mushroom tyrosinase | - |
Agaricus bisporus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 30 | - |
assay at | Agaricus bisporus |
1.11.1.14 | 30 | - |
assay at | Phanerodontia chrysosporium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | 0.12 | - |
veratryl alcohol | pH 6.5, 30°C | Agaricus bisporus | |
1.11.1.14 | 13.72 | - |
veratryl alcohol | pH 6.5, 30°C | Phanerodontia chrysosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.14 | 6.5 | - |
assay at | Agaricus bisporus |
1.11.1.14 | 6.5 | - |
assay at | Phanerodontia chrysosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.14 | heme | - |
Phanerodontia chrysosporium |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.11.1.14 | physiological function | the promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and Calpha-Cbeta bonds in 4-phenoxyphenol and guaiacyl glycerol-beta-guaiacyl ether (GGE) that are dimeric lignin model compounds. The promiscuous activity oxidizes lignin-related high redox potential substrates | Agaricus bisporus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.14 | 0.39 | - |
veratryl alcohol | pH 6.5, 30°C | Agaricus bisporus | |
1.11.1.14 | 3.88 | - |
veratryl alcohol | pH 6.5, 30°C | Phanerodontia chrysosporium |