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Literature summary extracted from

  • Casasampere, M.; Ordonez, Y.F.; Casas, J.; Fabrias, G.
    Dihydroceramide desaturase inhibitors induce autophagy via dihydroceramide-dependent and independent mechanisms (2017), Biochim. Biophys. Acta, 1861, 264-275 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.19.17 pharmacology the cells capacity to biosynthesize dihydroceramides must be taken into account in proautophagic Des1 inhibitors-including therapies Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.17 celecoxib CCX Homo sapiens
1.14.19.17 fenretinide
-
Homo sapiens
1.14.19.17 additional information in T98G and U87MG glioblastoma cell lines different Des1 inhibitory pro-autophagic compounds (DIPACS) exhibiting different cytotoxicities (CCX, PXD, resveratrol, gamma-tocotrienol and XM462), trigger autophagy via different pathways, both dihydroceramide-dependent and independent pathways Homo sapiens
1.14.19.17 phenoxodiol PXD Homo sapiens
1.14.19.17 resveratrol
-
Homo sapiens
1.14.19.17 tetrahydrocannabinol
-
Homo sapiens
1.14.19.17 XM462 an active-site directed Des1 inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.17 tetracosanoyl-dihydroceramide + reduced acceptor + O2 + 2 H+ Homo sapiens
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.17 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.17 glioblastoma cell
-
Homo sapiens
-
1.14.19.17 T-98G cell
-
Homo sapiens
-
1.14.19.17 U-87DND cell
-
Homo sapiens
-
1.14.19.17 U-87MG cell
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.17 N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphinganine + reduced acceptor + O2 + 2 H+
-
Homo sapiens ?
-
?
1.14.19.17 tetracosanoyl-dihydroceramide + reduced acceptor + O2 + 2 H+
-
Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.17 dihydroceramide desaturase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.17 37
-
in vivo assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.17 cytochrome b5
-
Homo sapiens

Expression

EC Number Organism Comment Expression
1.14.19.17 Homo sapiens gamma-tocotrienol causes downregulation of Des1 expression but is not inhibitory for the enzyme activity down

General Information

EC Number General Information Comment Organism
1.14.19.17 malfunction dihydroceramide desaturase 1 inhibitors activate autophagy via both dihydroceramide-dependent and independent pathways and the balance between the two pathways influences the final cell fate. Enzyme inhibitors celecoxib, resveratrol, phenoxodiol, and gamma-tocotrienol, but not gamma-tocopherol at 0.05 mM, promote an increase in dihydroceramide, dihydrosphingomyelins, and lactosyldihydroceramides in U-87MG and T-98G cell lines. Similar results are found with the active site-directed Des1 inhibitor XM462 Homo sapiens