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Literature summary extracted from

  • Smolik, A.C.; Bengez-Pudja, L.; Cheng, I.; Mascotti, D.P.
    Characterization of E. coli manganese superoxide dismutase binding to RNA and DNA (2014), Biochim. Biophys. Acta, 1844, 2251-2256 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.1 additional information
-
 additional information thermodynamics, dissociation constants, and kinetics of RNA binding by enzyme MnSOD at pH 6.0-7.5 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 additional information the bacterial manganese superoxide dismutase (MnSOD) localizes to the chromosomal portion of the cell Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ a MnSOD Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Escherichia coli
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 commercial preparation
-
 Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
 Escherichia coli O2 + H2O2
-
 ?
1.15.1.1 additional information equilibrium binding of Escherichia coli MnSOD to poly(U), poly(A), poly(C), poly(dU) and double-stranded (ds) DNA, overview. The polynucleotides bind to MnSOD in the following affinity hierarchy, poly(dU) N poly(U) N dsDNA N poly(A) N poly(C). The differences in the hierarchy are not large in magnitude as the poly(dU) bound with less than a 100fold higher affinity than poly(C). For each polynucleotide, Kobs decreases only slightly with increasing [K+], surprising for a relatively non-specific nucleic acid protein. There is either only one binding site shared by these polynucleotides or the larger site size occluded by poly(C) overlaps that of poly(U) and poly(A), but extends further on the protein Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 manganese superoxide dismutase
-
 Escherichia coli
1.15.1.1 MnSOD
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 6 7.5 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
1.15.1.1 physiological function the bacterial manganese superoxide dismutase (MnSOD) localizes to the chromosomal portion of the cell and impart protection from ionizing radiation to DNA. MnSOD can bind to RNA leads to the possibility that MnSOD may confer protection to RNA, as well Escherichia coli