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Literature summary extracted from

  • Zhang, X.; Eser, B.; Chanani, P.; Begley, T.; Ealick, S.
    Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases (2016), Biochemistry, 55, 1826-1838 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.60 expression in ERscherichia coli Methanocaldococcus jannaschii
2.4.2.60 expression in ERscherichia coli Methanocaldococcus igneus
2.4.2.60 expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.59 enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate Methanocaldococcus jannaschii
2.4.2.59 enzyme complexes with glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5 Methanotorris igneus
2.4.2.60 C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5 Saccharomyces cerevisiae
2.4.2.60 structure in complex with ADP-ribulose. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom Methanocaldococcus jannaschii
2.4.2.60 structure of mutant C205S with a bound glycine imine intermediate. Comparison with structures of Methanococcus jannaschii and Methanococcus igneus homologues Saccharomyces cerevisiae
2.4.2.60 structure with bound glycine imine intermediate and iron. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom Methanocaldococcus igneus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.60 C205S the mutant is catalytic in the presence of exogenous sulfide Saccharomyces cerevisiae
2.4.2.60 C207A inactive Saccharomyces cerevisiae
2.4.2.60 H237N the mutant only catalyzes the conversion of ADP-ribose to ADP-ribulose Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.59 Fe2+ dependent on Methanocaldococcus jannaschii
2.4.2.59 Fe2+ dependent on Methanotorris igneus
2.4.2.60 Fe2+ dependent on Saccharomyces cerevisiae
2.4.2.60 Iron
-
 Methanocaldococcus igneus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.59 NAD+ + glycine + sulfide Methanocaldococcus jannaschii
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide Methanotorris igneus
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide Methanocaldococcus jannaschii DSM 2661
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide Methanocaldococcus jannaschii ATCC 43067
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Saccharomyces cerevisiae
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.59 Methanocaldococcus jannaschii Q58018
-
-
2.4.2.59 Methanocaldococcus jannaschii ATCC 43067 Q58018
-
-
2.4.2.59 Methanotorris igneus F6BCS4
-
-
2.4.2.60 Methanocaldococcus igneus
-
-
-
2.4.2.60 Methanocaldococcus jannaschii Q58018
-
-
2.4.2.60 Methanocaldococcus jannaschii DSM 2661 Q58018
-
-
2.4.2.60 Saccharomyces cerevisiae
-
-
-
2.4.2.60 Saccharomyces cerevisiae P32318
-
-
2.4.2.60 Saccharomyces cerevisiae ATCC 204508 P32318
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.59 NAD+ + glycine + sulfide
-
 Methanocaldococcus jannaschii nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide
-
 Methanotorris igneus nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide
-
 Methanocaldococcus jannaschii DSM 2661 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.59 NAD+ + glycine + sulfide
-
 Methanocaldococcus jannaschii ATCC 43067 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
 ?
2.4.2.60 NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Saccharomyces cerevisiae nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.59 MJ0601
-
 Methanocaldococcus jannaschii
2.4.2.59 Thi4
-
 Methanocaldococcus jannaschii
2.4.2.59 Thi4
-
 Methanotorris igneus
2.4.2.60 Thi4
-
 Saccharomyces cerevisiae
2.4.2.60 Thi4
-
 Methanocaldococcus jannaschii
2.4.2.60 Thi4
-
 Methanocaldococcus igneus