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Literature summary extracted from

  • Madyastha, K.M.; Meehan, T.D.; Coscia, C.J.
    Characterization of a cytochrome P-450 dependent monoterpene hydroxylase from the higher plant Vinca rosea (1976), Biochemistry, 15, 1097-1102 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.83 dithiothreitol 3fold increase in activity in presence of dithiothreitol Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.83 4-chloromercuribenzoate 0.1 mM, 17% residual activity Catharanthus roseus
1.14.14.83 CO inhibition increases with increasing CO concentrations and is almost completely reversed with the aid of a light spurce between 420 and 450 nm Catharanthus roseus
1.14.14.83 iodoacetamide 10 mM, 87% residual activity Catharanthus roseus
1.14.14.83 Metyrapone 0.1 mM, 41% residual activity Catharanthus roseus
1.14.14.83 N-ethylmaleimide 1 mM, 58% residual activity Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.83 0.0055
-
 geraniol pH 7.2, 25°C Catharanthus roseus
1.14.14.83 0.011
-
 nerol pH 7.2, 25°C Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.83 membrane
-
 Catharanthus roseus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 (6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.83 solubilization by treatment with sodium cholate, Renex-30, or Lubrol-WX Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.83 seedling etiolated seedling Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus (6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.83 cytochrome P450
-
 Catharanthus roseus