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Literature summary extracted from

  • Eady, R.; Richardson, T.; Miller, R.; Hawkins, M.; Lowe, D.
    The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the Fe protein (1988), Biochem. J., 256, 189-196 .
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.2 Iron 4 mol of iron per mol of iron-subunit Azotobacter chroococcum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.2 62500
-
 gel filtration, iron-subunit Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum
-
 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum MCD1
-
 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.2 Azotobacter chroococcum P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-
1.18.6.2 Azotobacter chroococcum MCD1 P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.2 purification of the iron-subint Azotobacter chroococcum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.2 337
-
 formation of NH3, pH not specified in the publication, temperature not specified in the publication Azotobacter chroococcum
1.18.6.2 1211
-
 evolution of H2 under argon atmosphere, pH not specified in the publication, temperature not specified in the publication Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?
1.18.6.2 12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum MCD1 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.2 dimer 2 * 32000, SDS-PAGE, isolated iron-subunit Azotobacter chroococcum

Synonyms

EC Number Synonyms Comment Organism
1.18.6.2 vnfD alpha-chain Azotobacter chroococcum
1.18.6.2 vnfG delta-chain Azotobacter chroococcum
1.18.6.2 vnfK beta-chain Azotobacter chroococcum