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Literature summary extracted from

  • Lan, X.; Zeng, J.; Liu, K.; Zhang, F.; Bai, G.; Chen, M.; Liao, Z.; Huang, L.
    Comparison of two hyoscyamine 6beta-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida (2018), Biochem. Biophys. Res. Commun., 497, 25-31 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.11.11 synthesis overexpression of the enzyme (H6H) enhances the biosynthesis of anisodamine and scopolamine in hairy root cultures of Scopolia lurida, which should improve its commercial value. The tropane alkaloids anisodamine and and scopolamine are clinically used as anticholinergic agents Hyoscyamus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.11 overexpression in hairy root cultures of Scopolia lurida Hyoscyamus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.11 Hyoscyamus niger P24397
-
-
1.14.20.13 Anisodus luridus
-
-
-
1.14.20.13 Hyoscyamus niger P24397
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.13 root
-
 Hyoscyamus niger
-
1.14.20.13 root
-
 Anisodus luridus
-

Synonyms

EC Number Synonyms Comment Organism
1.14.11.11 H6H
-
 Hyoscyamus niger
1.14.11.11 hyoscyamine 6beta-hydroxylase
-
 Hyoscyamus niger

General Information

EC Number General Information Comment Organism
1.14.20.13 physiological function root cultures overexpressing the enzyme show remarkably elevated levels of scopolamine and anisodamine. Hyoscyamine 6beta-hydroxylases from both Scopolia lurida and Hyoscyamus niger promote anisodamine production at similar levels in Scopolia lurida root cultures. Hyoscyamus niger hyoscyamine 6beta-hydroxylase overexpressing root cultures have more scopolamine in them than Scopolia lurida hyoscyamine 6beta-hydroxylase-overexpressing root cultures Hyoscyamus niger
1.14.20.13 physiological function root cultures overexpressing the enzyme show remarkably elevated levels of scopolamine and anisodamine. Hyoscyamine 6beta-hydroxylases from both Scopolia lurida and Hyoscyamus niger promote anisodamine production at similar levels in Scopolia lurida root cultures. Hyoscyamus niger hyoscyamine 6beta-hydroxylase overexpressing root cultures have more scopolamine in them than Scopolia lurida hyoscyamine 6beta-hydroxylase-overexpressing root cultures Anisodus luridus