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Literature summary extracted from

  • Duncan, J.D.; Wallis, J.O.; Azari, M.R.
    Purification and properties of Aerococcus viridans lactate oxidase (1989), Biochem. Biophys. Res. Commun., 164, 919-926 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.2 Bromopyruvate irreversible inactivation Aerococcus viridans
1.1.3.2 D-lactate competitive Aerococcus viridans
1.1.3.2 DL-2-Hydroxybutanoate competitive Aerococcus viridans
1.1.3.2 glycolate competitive Aerococcus viridans
1.1.3.2 oxalate competitive Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.2 0.43
-
 (S)-lactate pH 7.0, 37°C Aerococcus viridans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.2 162000
-
 gel filtration Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.2 Aerococcus viridans Q44467
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.2
-
 Aerococcus viridans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.2 272
-
 pH 7.0, 37°C Aerococcus viridans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.2 (S)-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?
1.1.3.2 additional information enzyme is highly specific for L-lactate. No substrates: D-lactate, glycolate and DL-2-hydroxybutanoate Aerococcus viridans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.2 tetramer 4 * 44000, SDS-PAGE Aerococcus viridans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.2 FMN
-
 Aerococcus viridans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.2 1.2
-
 glycolate pH 7.0, 37°C Aerococcus viridans
1.1.3.2 2.9
-
 D-lactate pH 7.0, 37°C Aerococcus viridans
1.1.3.2 5.3
-
 oxalate pH 7.0, 37°C Aerococcus viridans
1.1.3.2 15
-
 DL-2-Hydroxybutanoate pH 7.0, 37°C Aerococcus viridans