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Literature summary extracted from

  • Laskoski, K.; Santos, A.R.; Bonatto, A.C.; Pedrosa, F.O.; Souza, E.M.; Huergo, L.F.
    In vitro characterization of the NAD+ synthetase NadE1 from Herbaspirillum seropedicae (2016), Arch. Microbiol., 198, 307-313 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.1 gene nadE1, sequence comparisons, the nadE1 gene is linked to glnB encoding the signal transduction protein GlnB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Herbaspirillum seropedicae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.1 additional information
-
 additional information Michaelis-Menten and Hanes-Woolf kinetics, kinetic analysis Herbaspirillum seropedicae
6.3.5.1 0.01
-
 deamido-NAD+ pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae
6.3.5.1 0.031
-
 ATP pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae
6.3.5.1 0.365
-
 L-glutamine pH 8.5, 30°C, recombinant His-tagged enzyme Herbaspirillum seropedicae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.1 Mg2+ required Herbaspirillum seropedicae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.1 548000
-
 recombinant enzyme, gel filtration Herbaspirillum seropedicae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + L-glutamine + H2O Herbaspirillum seropedicae
-
 AMP + diphosphate + NAD+ + L-glutamate
-
 ?
6.3.5.1 ATP + deamido-NAD+ + L-glutamine + H2O Herbaspirillum seropedicae SmR1
-
 AMP + diphosphate + NAD+ + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.1 Herbaspirillum seropedicae D8IS59
-
-
6.3.5.1 Herbaspirillum seropedicae SmR1 D8IS59
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Herbaspirillum seropedicae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + L-glutamine + H2O
-
 Herbaspirillum seropedicae AMP + diphosphate + NAD+ + L-glutamate
-
 ?
6.3.5.1 ATP + deamido-NAD+ + L-glutamine + H2O
-
 Herbaspirillum seropedicae SmR1 AMP + diphosphate + NAD+ + L-glutamate
-
 ?
6.3.5.1 additional information enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 Herbaspirillum seropedicae ?
-
 ?
6.3.5.1 additional information enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 Herbaspirillum seropedicae SmR1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.1 octamer 8 * 70000-75000, recombinant enzyme, SDS-PAGE, sequence calculation, and gel filtration Herbaspirillum seropedicae

Synonyms

EC Number Synonyms Comment Organism
6.3.5.1 glutamine-dependent NAD synthetase
-
 Herbaspirillum seropedicae
6.3.5.1 glutamine-dependent NadEGln
-
 Herbaspirillum seropedicae
6.3.5.1 Hsero_1894
-
 Herbaspirillum seropedicae
6.3.5.1 NadE1
-
 Herbaspirillum seropedicae
6.3.5.1 nicotinamide adenine dinucleotide synthetase enzyme
-
 Herbaspirillum seropedicae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.1 30
-
 assay at Herbaspirillum seropedicae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.1 8.5
-
 assay at Herbaspirillum seropedicae

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.1 ATP
-
 Herbaspirillum seropedicae