Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schäfer, T.; Schönheit, P.
    Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus involvement of reactions of the Embden-Meyerhof pathway (1993), Arch. Microbiol., 159, 354-363 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.59 0.025
-
 NADPH 50°C, pH not specified in the publication Pyrococcus furiosus
1.2.1.59 0.05
-
 NADP+ 50°C, pH not specified in the publication Pyrococcus furiosus
1.2.1.59 1.4
-
 NADH 50°C, pH not specified in the publication Pyrococcus furiosus
1.2.1.59 1.6
-
 NAD+ 50°C, pH not specified in the publication Pyrococcus furiosus
2.7.2.3 2
-
 3-phospho-D-glycerate 50°C, pH not specified in the publication Pyrococcus furiosus
2.7.2.3 2.7
-
 ATP 50°C, pH not specified in the publication Pyrococcus furiosus
2.7.9.2 0.07
-
 ATP 50°C, pH not specified in the publication Pyrococcus furiosus
4.1.1.31 2
-
 phosphoenolpyruvate 50°C, pH not specified in the publication Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 Mg2+ dependent on Mg2+, saturated at 5 mM concentration Pyrococcus furiosus
2.7.9.2 Mg2+ required Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.59 Pyrococcus furiosus P61879
-
-
2.7.2.3 Pyrococcus furiosus P61883
-
-
2.7.9.2 Pyrococcus furiosus P42850
-
-
4.1.1.31 Pyrococcus furiosus Q8TZL5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.59 0.009
-
 50°C, pH not specified in the publication, cofactor: NAD+, in presence of arsenate Pyrococcus furiosus
1.2.1.59 0.019
-
 50°C, pH not specified in the publication, cofactor: NADP+, in presence of arsenate Pyrococcus furiosus
1.2.1.59 0.035
-
 50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH Pyrococcus furiosus
1.2.1.59 0.06
-
 50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH Pyrococcus furiosus
2.7.2.3 0.011
-
 50°C, pH not specified in the publication Pyrococcus furiosus
2.7.2.3 0.022
-
 60°C, pH not specified in the publication Pyrococcus furiosus
2.7.9.2 0.05
-
 50°C, pH not specified in the publication Pyrococcus furiosus
4.1.1.31 0.0018
-
 50°C, pH not specified in the publication Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrococcus furiosus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NADP+ NADP+ is the preferred cofactor Pyrococcus furiosus 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Pyrococcus furiosus ADP + 3-phospho-D-glyceroyl phosphate
-
 ?
2.7.9.2 ATP + pyruvate + H2O
-
 Pyrococcus furiosus AMP + phosphoenolpyruvate + phosphate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Pyrococcus furiosus phosphate + oxaloacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.59 glyceraldehyde-3-phosphate dehydrogenase
-
 Pyrococcus furiosus
2.7.2.3 phosphoglycerate kinase
-
 Pyrococcus furiosus
2.7.9.2 PEP synthetase
-
 Pyrococcus furiosus
2.7.9.2 phosphoenolpyruvate synthetase
-
 Pyrococcus furiosus
4.1.1.31 PEP-carboxylase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.59 50
-
 assay at Pyrococcus furiosus
2.7.2.3 50
-
 assay at Pyrococcus furiosus
2.7.9.2 50
-
 assay at Pyrococcus furiosus
4.1.1.31 50
-
 assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.59 NAD+
-
 Pyrococcus furiosus
1.2.1.59 NADP+ NADP+ is the preferred cofactor Pyrococcus furiosus

Expression

EC Number Organism Comment Expression
1.2.1.59 Pyrococcus furiosus induced 10fold under growth conditions which require gluconeogenesis up