EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.14.9 | synthesis | investigation of a flask-scale production of caffeic acid from p-coumaric acid as the model reaction for HpaBC-catalyzed synthesis of hydroxycinnamic acids. Since the initial concentrations of the substrate p-coumaric acid higher than 40 mM markedly inhibits its 4-hydroxyphenylacetate 3-hydroxylases-catalyzed oxidation, the reaction is carried out by repeatedly adding 20 mM of this substrate to the reaction mixture. Furthermore, by using the whole-cell catalyst in the presence of glycerol, the experimental setup achieves the high-yield production of caffeic acid, i.e., 56.6 mM (10.2 g/l) within 24 h. These catalytic activities of 4-hydroxyphenylacetate 3-hydroxylases will provide an easy and environment-friendly synthetic approach to hydroxycinnamic acids | Pseudomonas aeruginosa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.9 | expression in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.9 | p-coumaric acid | substrate inhibition at concentrations higher than 40 mM | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.9 | Pseudomonas aeruginosa | Q9HWT7 and Q9HWT6 | hpaB: Q9HWT7, hpaC: Q9HWT6. The enzyme consists of a reductase component HpaC and an oxygenase component HpaB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.9 | 3-(3,4-dihydroxyphenyl)-propanoic acid + FADH2 + O2 | low activity | Pseudomonas aeruginosa | 3-(3,4,5-trihydroxyphenyl)-propanoic acid + FAD + H2O | - |
? | |
1.14.14.9 | 3-(4-hydroxyphenyl)-propanoic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3-(3,4-dihydroxyphenyl)-propanoic acid + FAD + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3,4-dihydroxyphenylacetate + FAD + H2O | - |
? | |
1.14.14.9 | caffeic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3,4,5-trihydroxycinnamic acid + FAD + H2O | - |
? | |
1.14.14.9 | coniferaldehyde + FADH2 + O2 | - |
Pseudomonas aeruginosa | 5-hydroxyconiferaldehyde + FAD + H2O | - |
? | |
1.14.14.9 | ferulic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 5-hydroxyferulic acid + FAD + H2O | - |
? | |
1.14.14.9 | additional information | no or very low oxidation activity toward cinnamic acid, o-coumaric acid and m-coumaric acid | Pseudomonas aeruginosa | ? | - |
? | |
1.14.14.9 | p-coumaric acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | caffeic acid + FAD + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.9 | 4-hydroxyphenylacetate 3-hydroxylase | - |
Pseudomonas aeruginosa |
1.14.14.9 | HPA 3-hydroxylase | - |
Pseudomonas aeruginosa |
1.14.14.9 | hpaBC | - |
Pseudomonas aeruginosa |
1.14.14.9 | HPAH | - |
Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.9 | FAD | - |
Pseudomonas aeruginosa |