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Literature summary extracted from

  • Franceus, J.; Pinel, D.; Desmet, T.
    Glucosylglycerate phosphorylase, an enzyme with novel specificity involved in compatible solute metabolism (2017), Appl. Environ. Microbiol., 83, e01434-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.352 expressed in Escherichia coli Meiothermus silvanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.352 2.5
-
 phosphate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 2.6
-
 D-glycerate at pH 6.5 and 42°C Meiothermus silvanus
2.4.1.352 3.5
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 8.1
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.352 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate Meiothermus silvanus
-
 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
2.4.1.352 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate Meiothermus silvanus ATCC 700542
-
 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
2.4.1.352 alpha-D-glucopyranose 1-phosphate + D-glycerate Meiothermus silvanus
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r
2.4.1.352 alpha-D-glucopyranose 1-phosphate + D-glycerate Meiothermus silvanus ATCC 700542
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.352 Meiothermus silvanus D7BAR0
-
-
2.4.1.352 Meiothermus silvanus ATCC 700542 D7BAR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.352 Ni-NTA column chromatography Meiothermus silvanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.352 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 Meiothermus silvanus alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
2.4.1.352 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 Meiothermus silvanus ATCC 700542 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 r
2.4.1.352 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 Meiothermus silvanus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r
2.4.1.352 alpha-D-glucopyranose 1-phosphate + D-glycerate
-
 Meiothermus silvanus ATCC 700542 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.352 GGaP
-
 Meiothermus silvanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.352 42
-
-
 Meiothermus silvanus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.352 0.83
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 0.98
-
 phosphate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 96
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus
2.4.1.352 128
-
 D-glycerate at pH 6.5 and 42°C Meiothermus silvanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.352 6
-
 optimum pH in phosphorolytic direction Meiothermus silvanus
2.4.1.352 6.5
-
 optimum pH in synthetic direction Meiothermus silvanus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.352 0.24
-
 2-O-(alpha-D-glucopyranosyl)-D-glycerate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 0.4
-
 phosphate at pH 6.0 and 42°C Meiothermus silvanus
2.4.1.352 11.8
-
 alpha-D-glucopyranose 1-phosphate at pH 6.5 and 42°C Meiothermus silvanus
2.4.1.352 49.4
-
 D-glycerate at pH 6.5 and 42°C Meiothermus silvanus