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Literature summary extracted from

  • Birke, J.; Röther, W.; Jendrossek, D.
    RoxB is a novel type of rubber oxygenase that combines properties of rubber oxygenase RoxA and latex clearing protein (Lcp) (2017), Appl. Environ. Microbiol., 83, e00721 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.11.87 degradation enzyme exerts a synergistic effect on the efficiency of polyisoprene cleavage by rubber oxidase RoxA, EC 1.13.11.85 Xanthomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.87 Diethyl dithiocarbamate
-
 Xanthomonas sp.
1.13.11.87 imidazole
-
 Xanthomonas sp.
1.13.11.87 additional information not inhibitory: EDTA up to 10 mM, carbon monoxide Xanthomonas sp.
1.13.11.87 Pyridine
-
 Xanthomonas sp.
1.13.11.87 SDS partial inhibition Xanthomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.87 Xanthomonas sp. A0A1S6Q8F9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.87 proteolytic modification calculated molecular mass is 73800 Da for precursor protein, and 70300 Da for mature protein Xanthomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.87 4.5
-
 pH 7.0, 23°C Xanthomonas sp.
1.13.11.87 5.7
-
 pH 7.0, 30°C Xanthomonas sp.
1.13.11.87 6.4
-
 pH 7.0, 37°C Xanthomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.87 cis-1,4-polyisoprene + n O2
-
 Xanthomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.87 ? x * 73800, calculated for precursor protein, x * 70300, calculated for mature protein, x * 70300, SDS-PAGE Xanthomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.87 RoxB
-
 Xanthomonas sp.
1.13.11.87 rubber oxygenase B
-
 Xanthomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.87 6 8
-
 Xanthomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.87 heme presence of 2 c-type hemes, presence of two c-type heme binding motifs (C192HAC195H and C391ASC394H) Xanthomonas sp.

Expression

EC Number Organism Comment Expression
1.13.11.87 Xanthomonas sp. expression is upregulated during growth on polyisoprene up

General Information

EC Number General Information Comment Organism
1.13.11.87 physiological function synergistic effect of the simultaneous presence of RoxAXsp and RoxBXsp on the efficiency of polyisoprene cleavage Xanthomonas sp.