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Literature summary extracted from

  • Birke, J.; Röther, W.; Jendrossek, D.
    Latex clearing protein (Lcp) of Streptomyces sp. strain K30 is a b-type cytochrome and differs from rubber oxygenase A (RoxA) in its biophysical properties (2015), Appl. Environ. Microbiol., 81, 3793-3799 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.87 expression in Escherichia coli Streptomyces sp. K30

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.87 1,10-phenanthroline 1 mM, 20% inhibition Streptomyces sp. K30
1.13.11.87 diethyldithiocarbamate 1 mM, 56% inhibition Streptomyces sp. K30
1.13.11.87 Diethylthiocarbamate 1 mM, 56% inhibition Streptomyces sp. K30
1.13.11.87 Dithionite 1 mM, 15% inhibition Streptomyces sp. K30
1.13.11.87 ethyl xanthogenate 1 mM, 7% inhibition Streptomyces sp. K30
1.13.11.87 imidazole 1 mM, 48% inhibition Streptomyces sp. K30

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.87 Fe3+ purified Lcp contains an oxidized heme-Fe3+ Streptomyces sp. K30

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.87 48000
-
SDS-PAGE Streptomyces sp. K30

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.85 cis-1,4-polyisoprene + O2 Xanthomonas sp. 35Y
-
(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.85 Xanthomonas sp. Q7X0P3
-
-
1.13.11.85 Xanthomonas sp. 35Y Q7X0P3
-
-
1.13.11.87 Streptomyces sp. K30 Q3L8N0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.85
-
Xanthomonas sp. 35Y
1.13.11.87
-
Streptomyces sp. K30

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.85 4.6
-
37°C, pH 7.0 Xanthomonas sp.
1.13.11.87 1.5
-
23°C. pH 7.0 Streptomyces sp. K30
1.13.11.87 4.6
-
37°C, pH 7.0 Streptomyces sp. K30

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.85 cis-1,4-polyisoprene + n O2
-
Xanthomonas sp. n 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al
-
?
1.13.11.85 cis-1,4-polyisoprene + O2
-
Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
?
1.13.11.87 cis-1,4-polyisoprene + O2
-
Streptomyces sp. K30 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.87 ? x * 48000, SDS-PAGE Streptomyces sp. K30

Synonyms

EC Number Synonyms Comment Organism
1.13.11.85 roxA
-
Xanthomonas sp. 35Y
1.13.11.85 rubber oxygenase A
-
Xanthomonas sp. 35Y
1.13.11.87 latex clearing protein
-
Streptomyces sp. K30
1.13.11.87 Lcp
-
Streptomyces sp. K30

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.85 54.3
-
melting point Xanthomonas sp.
1.13.11.85 54.3
-
melting point Xanthomonas sp. 35Y
1.13.11.87 61.5
-
melting point Streptomyces sp. K30

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.85 heme covalently bound heme firmly binds a dioxygen molecule Xanthomonas sp.
1.13.11.85 heme c-type heme containing enzyme Xanthomonas sp. 35Y
1.13.11.87 heme the enzyme is a member of the b-type cytochrome family Streptomyces sp. K30