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Literature summary extracted from

  • Montero-Moran, G.M.; Sampedro, J.G.; Saab-Rincon, G.; Cervantes-Gonzalez, M.A.; Huerta-Ocampo, J.A.; De Leon-Rodriguez, A.; Barba de la Rosa, A.P.
    Biochemical and molecular characterization of a novel Cu/Zn superoxide dismutase from Amaranthus hypochondriacus L. an intrinsically disordered protein (2015), Appl. Biochem. Biotechnol., 176, 2328-2345 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 AhSOD DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Amaranthus hypochondriacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2
-
 Amaranthus hypochondriacus
1.15.1.1 KCN
-
 Amaranthus hypochondriacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu/ZnSOD, highly conserved amino acid residues are involved in Cu/Zn binding Amaranthus hypochondriacus
1.15.1.1 additional information enzyme affinities for copper, zinc, and nickel, overview Amaranthus hypochondriacus
1.15.1.1 Zn2+ a Cu/ZnSOD, highly conserved amino acid residues are involved in Cu/Zn binding Amaranthus hypochondriacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 28500
-
 recombinant His-tagged enzyme, gel filtration Amaranthus hypochondriacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Amaranthus hypochondriacus
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Amaranthus hypochondriacus F6JRN6 cv. Nutrisol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis, anion exchange chromatography and dialysis, and ultrafiltration Amaranthus hypochondriacus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 root
-
 Amaranthus hypochondriacus
-
1.15.1.1 seedling
-
 Amaranthus hypochondriacus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
 Amaranthus hypochondriacus O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 15600, about, sequence calculation Amaranthus hypochondriacus
1.15.1.1 More enzyme structural analysis by circular dichroism analysis, recombinnat AhSOD is an intrinsically disorder enzyme, sequence comparisons and three-dimensional model Amaranthus hypochondriacus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 AhSOD
-
 Amaranthus hypochondriacus
1.15.1.1 Cu/Zn superoxide dismutase
-
 Amaranthus hypochondriacus
1.15.1.1 Cu/ZnSOD
-
 Amaranthus hypochondriacus
1.15.1.1 SOD
-
 Amaranthus hypochondriacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 37
-
 assay at Amaranthus hypochondriacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 8
-
 assay at Amaranthus hypochondriacus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Amaranthus hypochondriacus sequence calculation
-
 5.4