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Literature summary extracted from

  • Frese, M.; Sewald, N.
    Enzymatic halogenation of tryptophan on a gram scale (2015), Angew. Chem. Int. Ed. Engl., 54, 298-301 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.9 recombinant expression in Escherichia coli Lentzea aerocolonigenes

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.9 additional information for optimization of the enzymatic regioselective halogenation process, a method that is easily scalable up to gram scale is established. An active FAD-dependent halogenase is co-immobilized with all auxiliary enzymes necessary for halogenation, formation of cross-linked enzyme aggregates (CLEAs) might stabilize its quaternary structure Lentzea aerocolonigenes

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.9 Lentzea aerocolonigenes Q8KHZ8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.9 5-hydroxy-L-tryptophan + FADH2 + chloride + O2 + H+ regioselective halogenation, recombinant enzyme Lentzea aerocolonigenes 7-chloro-5-hydroxy-L-tryptophan + FAD + 2 H2O
-
?
1.14.19.9 D-tryptophan + FADH2 + bromide + O2 + H+ regioselective halogenation, recombinant enzyme Lentzea aerocolonigenes 7-bromo-D-tryptophan + FAD + 2 H2O
-
?
1.14.19.9 L-tryptophan + FADH2 + bromide + O2 + H+ best substrates, regioselective halogenation, recombinant enzyme Lentzea aerocolonigenes 7-bromo-L-tryptophan + FAD + 2 H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.19.9 dimer
-
Lentzea aerocolonigenes

Synonyms

EC Number Synonyms Comment Organism
1.14.19.9 flavin-dependent tryptophan halogenase
-
Lentzea aerocolonigenes
1.14.19.9 RebH
-
Lentzea aerocolonigenes
1.14.19.9 Trp-7-halogenase
-
Lentzea aerocolonigenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.9 25
-
assay at Lentzea aerocolonigenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.9 7.4
-
assay at Lentzea aerocolonigenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.9 FAD FAD-dependent halogenase Lentzea aerocolonigenes
1.14.19.9 NAD+
-
Lentzea aerocolonigenes