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Literature summary extracted from

  • Wu, X.; Zhou, F.; Tu, G.; Zou, X.
    Gene cloning, expression and characterization of malate-CoA ligase in the polymerization pathway of polymalic acid from Aureobasidium pullulans (2014), Acta Microbiol. Sin., 54, 919-925 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.9 expressed in Escherichia coli BL21(DE3) cells Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.9 ATP high substrate concentrations of ATP inhibit the enzyme activity Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.9 ATP + malate + CoA Aureobasidium pullulans
-
 ADP + phosphate + malyl-CoA
-
 ?
6.2.1.9 ATP + malate + CoA Aureobasidium pullulans CCTCC M2012223
-
 ADP + phosphate + malyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.9 Aureobasidium pullulans
-
-
-
6.2.1.9 Aureobasidium pullulans CCTCC M2012223
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.9 Ni-NTA column chromatography Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.9 ATP + butyrate + CoA
-
 Aureobasidium pullulans ADP + phosphate + butyryl-CoA
-
 ?
6.2.1.9 ATP + butyrate + CoA
-
 Aureobasidium pullulans CCTCC M2012223 ADP + phosphate + butyryl-CoA
-
 ?
6.2.1.9 ATP + malate + CoA
-
 Aureobasidium pullulans ADP + phosphate + malyl-CoA
-
 ?
6.2.1.9 ATP + malate + CoA
-
 Aureobasidium pullulans CCTCC M2012223 ADP + phosphate + malyl-CoA
-
 ?
6.2.1.9 ATP + malonate + CoA
-
 Aureobasidium pullulans ADP + phosphate + malonyl-CoA
-
 ?
6.2.1.9 ATP + malonate + CoA
-
 Aureobasidium pullulans CCTCC M2012223 ADP + phosphate + malonyl-CoA
-
 ?
6.2.1.9 ATP + oxalate + CoA
-
 Aureobasidium pullulans ADP + phosphate + oxalyl-CoA
-
 ?
6.2.1.9 ATP + oxalate + CoA
-
 Aureobasidium pullulans CCTCC M2012223 ADP + phosphate + oxalyl-CoA
-
 ?
6.2.1.9 ATP + oxaloacetate + CoA
-
 Aureobasidium pullulans ADP + phosphate + oxaloacetyl-CoA
-
 ?
6.2.1.9 ATP + oxaloacetate + CoA
-
 Aureobasidium pullulans CCTCC M2012223 ADP + phosphate + oxaloacetyl-CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.9 25
-
-
 Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.9 8
-
-
 Aureobasidium pullulans

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.9 ATP
-
 Aureobasidium pullulans