BRENDA - Enzyme Database

Gene cloning, expression and characterization of malate-CoA ligase in the polymerization pathway of polymalic acid from Aureobasidium pullulans

Wu, X.; Zhou, F.; Tu, G.; Zou, X.; Acta Microbiol. Sin. 54, 919-925 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.2.1.9
expressed in Escherichia coli BL21(DE3) cells
Aureobasidium pullulans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.9
ATP
high substrate concentrations of ATP inhibit the enzyme activity
Aureobasidium pullulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.9
ATP + malate + CoA
Aureobasidium pullulans
-
ADP + phosphate + malyl-CoA
-
-
?
6.2.1.9
ATP + malate + CoA
Aureobasidium pullulans CCTCC M2012223
-
ADP + phosphate + malyl-CoA
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.9
Aureobasidium pullulans
-
-
-
6.2.1.9
Aureobasidium pullulans CCTCC M2012223
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.9
Ni-NTA column chromatography
Aureobasidium pullulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.9
ATP + butyrate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + butyryl-CoA
-
-
-
?
6.2.1.9
ATP + butyrate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + butyryl-CoA
-
-
-
?
6.2.1.9
ATP + malate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + malyl-CoA
-
-
-
?
6.2.1.9
ATP + malate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + malyl-CoA
-
-
-
?
6.2.1.9
ATP + malonate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + malonyl-CoA
-
-
-
?
6.2.1.9
ATP + malonate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + malonyl-CoA
-
-
-
?
6.2.1.9
ATP + oxalate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + oxalyl-CoA
-
-
-
?
6.2.1.9
ATP + oxalate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + oxalyl-CoA
-
-
-
?
6.2.1.9
ATP + oxaloacetate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + oxaloacetyl-CoA
-
-
-
?
6.2.1.9
ATP + oxaloacetate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + oxaloacetyl-CoA
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.9
25
-
-
Aureobasidium pullulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.9
8
-
-
Aureobasidium pullulans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.2.1.9
ATP
-
Aureobasidium pullulans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.9
expressed in Escherichia coli BL21(DE3) cells
Aureobasidium pullulans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.2.1.9
ATP
-
Aureobasidium pullulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.9
ATP
high substrate concentrations of ATP inhibit the enzyme activity
Aureobasidium pullulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.9
ATP + malate + CoA
Aureobasidium pullulans
-
ADP + phosphate + malyl-CoA
-
-
?
6.2.1.9
ATP + malate + CoA
Aureobasidium pullulans CCTCC M2012223
-
ADP + phosphate + malyl-CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.9
Ni-NTA column chromatography
Aureobasidium pullulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.9
ATP + butyrate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + butyryl-CoA
-
-
-
?
6.2.1.9
ATP + butyrate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + butyryl-CoA
-
-
-
?
6.2.1.9
ATP + malate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + malyl-CoA
-
-
-
?
6.2.1.9
ATP + malate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + malyl-CoA
-
-
-
?
6.2.1.9
ATP + malonate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + malonyl-CoA
-
-
-
?
6.2.1.9
ATP + malonate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + malonyl-CoA
-
-
-
?
6.2.1.9
ATP + oxalate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + oxalyl-CoA
-
-
-
?
6.2.1.9
ATP + oxalate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + oxalyl-CoA
-
-
-
?
6.2.1.9
ATP + oxaloacetate + CoA
-
743968
Aureobasidium pullulans
ADP + phosphate + oxaloacetyl-CoA
-
-
-
?
6.2.1.9
ATP + oxaloacetate + CoA
-
743968
Aureobasidium pullulans CCTCC M2012223
ADP + phosphate + oxaloacetyl-CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.9
25
-
-
Aureobasidium pullulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.9
8
-
-
Aureobasidium pullulans