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Literature summary extracted from

  • Chen, M.; Nakazawa, Y.; Kubo, Y.; Asano, N.; Kato, K.; Tanaka, I.; Yao, M.
    Crystallographic analysis of a subcomplex of the transsulfursome with tRNA for Cys-tRNACys synthesis (2016), Acta Crystallogr. Sect. F, 72, 569-572 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.27 expressed in Escherichia coli B834(DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.73 crystal structure of a subcomplex of the transsulfursome with tRNACys (SepCysS-SepCysE-tRNACys), which is involved in the second reaction step of the indirect pathway, to 2.6 A resolution. Space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1 A. Two heterodimers of a synthase SepCysS dimer, an enhancer SepCysE dimer and one tRNACys are observed in the asymmetric unit Methanocaldococcus jannaschii
6.1.1.27 sitting drop vapor diffusion method, using 0.1 M MES pH 5.3, 2.37 M ammonium sulfate Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.73 Methanocaldococcus jannaschii Q59072
-
-
2.5.1.73 Methanocaldococcus jannaschii DSM 2661 Q59072
-
-
6.1.1.27 Methanocaldococcus jannaschii Q59054
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.27 HisTrap column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys
-
 Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.73 SepCysS
-
 Methanocaldococcus jannaschii
6.1.1.27 CysRS
-
 Methanocaldococcus jannaschii
6.1.1.27 Cysteinyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.27 O-phosphoseryl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.27 SepRS
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.27 ATP
-
 Methanocaldococcus jannaschii