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Literature summary extracted from

  • Huang, J.; Malhi, M.; Deneke, J.; Fraser, M.E.
    Structure of GTP-specific succinyl-CoA synthetase in complex with CoA (2015), Acta Crystallogr. Sect. F, 71, 1067-1071 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.4 expressed in Escherichia coli BL21(DE3) cells Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.4 in complex with CoA, hanging drop vapor diffusion method, using 1.6 M ammonium sulfate, 1 mM 2-mercaptoethanol, 50 mM sodium/potassium phosphate pH 7.0 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.4 GTP + succinate + CoA Sus scrofa
-
 GDP + phosphate + succinyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.4 Sus scrofa P53590
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.4 Ni-NTA column chromatography and Superdex 200 gel filtration Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.4 GTP + succinate + CoA
-
 Sus scrofa GDP + phosphate + succinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.4 heterodimer
-
 Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
6.2.1.4 GTP-specific succinyl-CoA synthetase
-
 Sus scrofa
6.2.1.4 GTPSCS
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.4 GTP
-
 Sus scrofa