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Literature summary extracted from

  • Huang, J.; Fraser, M.
    Structural basis for the binding of succinate to succinyl-CoA synthetase (2016), Acta Crystallogr. Sect. D, 72, 912-921 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.4 expressed in Escherichia coli BL21(DE3) cells Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.4 in complex with succinate, magnesium ions and CoA, hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 and 0.2 M magnesium formate Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.4 V330M the mutant exhibits an about 10fold higher Km value for succinate compared to the wild type enzyme Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.4 0.49
-
 succinate wild type enzyme, at pH 7.4 and 21°C Sus scrofa
6.2.1.4 5.1
-
 succinate mutant enzyme V330M, at pH 7.4 and 21°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.4 Mg2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.4 GTP + succinate + CoA Sus scrofa
-
 GDP + phosphate + succinyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.4 Sus scrofa P53590
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.4 ammonium sulfate precipitation, HiTrap Blue column chromatography, and Sephadex G-25 gel filtration Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.4 GTP + L-malate + CoA low activity Sus scrofa GDP + phosphate + L-malyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA
-
 Sus scrofa GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 additional information no activity with citrate, fumarate and oxaloacetate Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.4 GTP-specific succinyl-CoA synthetase
-
 Sus scrofa
6.2.1.4 GTPSCS
-
 Sus scrofa
6.2.1.4 SUCLG2
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.4 GTP
-
 Sus scrofa