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Literature summary extracted from

  • Sun, X.; Lin, Y.; Yuan, Q.; Yan, Y.
    Precursor-directed biosynthesis of 5-hydroxytryptophan using metabolically engineered E. coli (2015), ACS Synth. Biol., 4, 554-558 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.172 enzyme S5H is encoded in the salABCD gene cluster, which locates on the chromosome rather than on a mobile plasmid, functional recombinant expression in Escherichia coli Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.172 additional information precursor-directed biosynthesis of 5-hydroxytryptophan using metabolically engineered Escherichia coli and involving the salicylate 5-hydroxylase from Ralstonia eutropha strain H16 Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.172 salicylate + NADH + H+ + O2 Cupriavidus necator
-
 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.172 Cupriavidus necator Q0KB56
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.172 anthranilate + NADH + H+ + O2
-
 Cupriavidus necator 5-hydroxyanthranilate + NAD+ + H2O
-
 ?
1.14.13.172 additional information 2-aminobenzoate might be a substrate for the enzyme Cupriavidus necator ?
-
 ?
1.14.13.172 salicylate + NADH + H+ + O2
-
 Cupriavidus necator 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.172 nagG
-
 Cupriavidus necator
1.14.13.172 nagG oxygenase component Cupriavidus necator
1.14.13.172 S5H
-
 Cupriavidus necator
1.14.13.172 SalABCD
-
 Cupriavidus necator
1.14.13.172 SALD enzyme complex component Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.172 additional information SalABCD requires NAD(P)H as the cofactor instead of BH4 Cupriavidus necator
1.14.13.172 NADH
-
 Cupriavidus necator

General Information

EC Number General Information Comment Organism
1.14.13.172 physiological function enzyme S5H hydroxylates salicylate into gentisate and is involved in the degradation of aromatic compounds. SalD is an essential component of the S5H complex Cupriavidus necator