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Literature summary extracted from

  • Zarei, A.; Trobacher, C.P.; Shelp, B.J.
    Arabidopsis aldehyde dehydrogenase 10 family members confer salt tolerance through putrescine-derived 4-aminobutyrate (GABA) production (2016), Sci. Rep., 6, 35115 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.19 gene ataldh10A8, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana protoplast, recombinant expression of His-tagged enzyme in in Escherichia coli Arabidopsis thaliana
1.2.1.19 gene ataldh10A9, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana protoplast, recombinant expression of His-tagged enzyme in in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.19 additional information generation of a ataldh10A8 T-DNA-insertion mutant that has lower levels of GABA than wild-type plants in response to salinity treatment and are more prone to salinity stress Arabidopsis thaliana
1.2.1.19 additional information generation of a ataldh10A9 T-DNA-insertion mutant that has lower levels of GABA than wild-type plants in response to salinity treatment and are more prone to salinity stress Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.19 additional information
-
additional information Michaelis-Menten kinetics Arabidopsis thaliana
1.2.1.19 0.0132
-
NAD+ with 4-aminobutanal, pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.0144
-
4-aminobutanal pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.0173
-
4-aminobutanal pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.0208
-
NAD+ with 3-aminopropanal, pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.0251
-
3-aminopropanal pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.0736
-
NAD+ with 3-aminopropanal, pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.086
-
NAD+ with 4-aminobutanal, pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 0.46
-
3-aminopropanal pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.19 chloroplast AtALDH10A8 is cytosolic, although the N-terminal 140 amino acid sequence of AtALDH10A8 localizes to the plastid and to the leucoplast Arabidopsis thaliana 9507
-
1.2.1.19 cytosol AtALDH10A8 is cytosolic, although the N-terminal 140 amino acid sequence of AtALDH10A8 localizes to the plastid and to the leucoplast Arabidopsis thaliana 5829
-
1.2.1.19 peroxisome
-
Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.19 3-aminobutanal + NAD+ + H2O Arabidopsis thaliana best substrate 3-aminobutanoate + NADH + 2 H+
-
?
1.2.1.19 3-aminopropanal + NAD+ + H2O Arabidopsis thaliana best substrate 3-aminopropanoate + NADH + H+
-
?
1.2.1.19 4-aminobutanal + NAD+ + H2O Arabidopsis thaliana
-
4-aminobutanoate + NADH + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Arabidopsis thaliana Q9S795
-
-
1.2.1.19 Arabidopsis thaliana Q9STS1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 recombinant His-tagged enzyme from in Escherichia coli by affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.19 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 3-aminobutanal + NAD+ + H2O best substrate Arabidopsis thaliana 3-aminobutanoate + NADH + 2 H+
-
?
1.2.1.19 3-aminopropanal + NAD+ + H2O best substrate Arabidopsis thaliana 3-aminopropanoate + NADH + H+
-
?
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
Arabidopsis thaliana 4-aminobutanoate + NADH + 2 H+
-
?
1.2.1.19 additional information maximal activity and catalytic efficiency are obtained with NAD+ and 3-aminopropanal, followed by 4-aminobutanal, negligible activity is obtained with betaine aldehyde. The betaine-aldehyde dehydrogenase ADH10A8 acts as 4-aminobutanal dehydrogenase Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.19 ALDH10A8
-
Arabidopsis thaliana
1.2.1.19 ALDH10A9
-
Arabidopsis thaliana
1.2.1.19 AMADH
-
Arabidopsis thaliana
1.2.1.19 More cf. EC 1.2.1.8 Arabidopsis thaliana
1.2.1.19 NAD+-dependent aminoaldehyde dehydrogenase
-
Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.19 22
-
assay at room temperature Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 9.5
-
-
Arabidopsis thaliana
1.2.1.19 10.5
-
-
Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.19 6.5 11 the enzyme retains approximately 20% of its maximal substrate-limited, 3-aminopropanal-dependent activity at pH 7.5 and is inactive at pH 6.5 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+
-
Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.2.1.19 Arabidopsis thaliana AtALDH109 gene is expressed constitutively in Arabidopsis thaliana, although it has been reported to be weakly induced by salinity and dehydration up
1.2.1.19 Arabidopsis thaliana AtALDH10A8 gene is expressed constitutively in Arabidopsis thaliana, although it has been reported to be weakly induced by salinity and dehydration up

General Information

EC Number General Information Comment Organism
1.2.1.19 evolution the enzyme is a member of the aldehyde dehydrogenase 10 family Arabidopsis thaliana
1.2.1.19 malfunction root growth of single loss-of-function mutants is more sensitive to salinity than wild-type plants, and this is accompanied by reduced GABA accumulation Arabidopsis thaliana
1.2.1.19 physiological function the enzyme shows maximal activity and catalytic efficiency with NAD+ and 3-aminopropanal, followed by 4-aminobutanal. Negligible activity is obtained with betaine aldehyde. NAD+ reduction is accompanied by the production of gamma-aminobutyrate, GABA, and beta-alanine, respectively, with 4-aminobutanal and 3-aminopropanal as substrates Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.19 20
-
3-aminopropanal pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 40
-
NAD+ with 3-aminopropanal, pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 60
-
3-aminopropanal pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 60
-
NAD+ with 3-aminopropanal, pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 150
-
NAD+ with 4-aminobutanal, pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 260
-
NAD+ with 4-aminobutanal, pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 820
-
4-aminobutanal pH 9.5, 22°C, recombinant enzyme Arabidopsis thaliana
1.2.1.19 1030
-
4-aminobutanal pH 8.5, 22°C, recombinant enzyme Arabidopsis thaliana