EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | arsenate | best at 10 mM and up to 30 mM | Naegleria gruberi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.12 | GAPDH gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Naegleria gruberi |
1.2.1.107 | expressed in Escherichia coli BL21(DE3) cells | Naegleria gruberi |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.12 | purified recombinant N-terminally His-tagged enzyme, hanging drop vapor diffusion method, mixing of 10 mg/ml protein with reservoir solution containing either 12.9% w/v PEG 2000, 50 mM acetate buffer, pH 5.2, and 6.4% w/v PEG 200, or 2.5% w/v PEG 1000, 50 mM acetate buffer, pH 4.8, and 25.7% w/v PEG 2000 MME, 18°C, 3 days, X-ray diffraction structure determination and analysis at 2.40 A and 1.94 A resolutions, respectively | Naegleria gruberi |
1.2.1.107 | hanging drop vapor diffusion method, using either 12.9% (w/w) PEG 2000, 50 mM acetate buffer (pH 5.2) and 6.4% (w/v) PEG 200 or2.5% (w/v) PEG 1000, 50 mM acetate buffer (pH 4.8) and 25.7% (w/v) PEG 2000 MME | Naegleria gruberi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | additional information | - |
additional information | the enzyme presents allosteric positive cooperativity for substrates NAD+ and D-glyceraldehyde 3-phosphate, kinetic analysis, overview | Naegleria gruberi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Naegleria gruberi | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Naegleria gruberi | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.12 | Naegleria gruberi | - |
nonpathogenic, free-living amoeba | - |
1.2.1.107 | Naegleria gruberi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.12 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickell affinity chromatography, dialysis, and gel filtration | Naegleria gruberi |
1.2.1.107 | nickel affinity column chromatography and Superdex 75 gel filtration | Naegleria gruberi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Naegleria gruberi | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Naegleria gruberi | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.12 | GAPDH | - |
Naegleria gruberi |
1.2.1.12 | glyceraldehyde-3-phosphate dehydrogenase | - |
Naegleria gruberi |
1.2.1.107 | GAPDH | - |
Naegleria gruberi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 25 | - |
- |
Naegleria gruberi |
1.2.1.107 | 25 | - |
- |
Naegleria gruberi |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 10 | 35 | activity range, profile overview | Naegleria gruberi |
1.2.1.107 | 20 | 30 | high activity between 20 and 30°C | Naegleria gruberi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 8 | - |
- |
Naegleria gruberi |
1.2.1.107 | 8 | - |
- |
Naegleria gruberi |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 6 | 10 | activity range, profile overview | Naegleria gruberi |
1.2.1.107 | 7.5 | 8.5 | high activity between pH 7.5 and 8.5 | Naegleria gruberi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | NAD+ | - |
Naegleria gruberi | |
1.2.1.107 | NAD+ | - |
Naegleria gruberi |