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Literature summary extracted from

  • Fernandes, P.; Aldeborgh, H.; Carlucci, L.; Walsh, L.; Wasserman, J.; Zhou, E.; Lefurgy, S.; Mundorff, E.
    Alteration of substrate specificity of alanine dehydrogenase (2015), Protein Eng. Des. Sel., 28, 29-35 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1
-
 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.1 F94S mutation alters its substrate specificity pattern, enabling activity toward a range of larger amino acids Mycobacterium tuberculosis
1.4.1.1 F94S/Y117L mutant shows improved activity toward hydrophobic amino acids Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 24
-
 L-serine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 53
-
 L-alanine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Mycobacterium tuberculosis P9WQB1
-
-
1.4.1.1 Mycobacterium tuberculosis H37Rv P9WQB1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-2-amino-4-pentenoate + H2O + NAD+
-
 Mycobacterium tuberculosis 2-oxo-4-pentenoate + NH3 + NADH
-
 ?
1.4.1.1 L-2-amino-4-pentenoate + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv 2-oxo-4-pentenoate + NH3 + NADH
-
 ?
1.4.1.1 L-alanine + H2O + NAD+
-
 Mycobacterium tuberculosis pyruvate + NH3 + NADH + H+
-
 ?
1.4.1.1 L-alanine + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv pyruvate + NH3 + NADH + H+
-
 ?
1.4.1.1 L-homophenylalanine + H2O + NADH + H+
-
 Mycobacterium tuberculosis 2-oxo-4-phenylbutanoate + NH3 + NAD+
-
 ?
1.4.1.1 L-homoserine + H2O + NAD+
-
 Mycobacterium tuberculosis 4-hydroxy-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 L-leucine + H2O + NAD+
-
 Mycobacterium tuberculosis 4-methyl-2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-methionine + H2O + NAD+
-
 Mycobacterium tuberculosis 4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 L-norleucine + H2O + NADH + H+
-
 Mycobacterium tuberculosis 2-oxohexanoate + NH3 + NAD+
-
 ?
1.4.1.1 L-norvaline + H2O + NAD+
-
 Mycobacterium tuberculosis 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-norvaline + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-serine + H2O + NAD+
-
 Mycobacterium tuberculosis 3-hydroxypyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-serine + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv 3-hydroxypyruvate + NH3 + NADH
-
 ?
1.4.1.1 L-valine + H2O + NAD+
-
 Mycobacterium tuberculosis 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 L-valine + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.1 0.24
-
 L-serine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 7.5
-
 L-alanine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.1 0.00035
-
 L-alanine mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0004
-
 L-norvaline wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.00088
-
 L-leucine mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0015
-
 L-2-amino-4-pentenoate wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0016
-
 L-methionine mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0017
-
 L-norvaline mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0022
-
 L-alanine mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0026
-
 L-methionine mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0027
-
 L-leucine mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.004
-
 L-2-amino-4-pentenoate mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.0046
-
 L-valine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.005
-
 L-2-amino-4-pentenoate mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.006
-
 L-norleucine mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.01
-
 L-serine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.013
-
 L-norleucine mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.015
-
 L-homoserine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.019
-
 L-homophenylalanine mutant F94S, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.037
-
 L-homophenylalanine mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 0.04
-
 L-norvaline mutant F94S/Y117L, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis
1.4.1.1 1.4
-
 L-alanine wild-type, pH 10.2, temperature not specified in the publication Mycobacterium tuberculosis