Any feedback?
Literature summary extracted from
- Heterologous production of extreme alkaline thermostable NAD+-dependent formate dehydrogenase with wide-range pH activity from Myceliophthora thermophila (2017), Process Biochem., 61, 110-118 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | expressed in Escherichia coli BL21(DE3) cells | Thermothelomyces thermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.003 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus |  |
| 1.17.1.9 | 0.4 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 1.2 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 7.2 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.9 | Thermothelomyces thermophilus | G2QB71 | - |
- |
| 1.17.1.9 | Thermothelomyces thermophilus ATCC 42464 | G2QB71 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | His-Trap column chromatography | Thermothelomyces thermophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.17.1.9 | -20°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity | Thermothelomyces thermophilus |
| 1.17.1.9 | -80°C, glycine buffer, pH 10.5, 30 days, 50% loss of activity | Thermothelomyces thermophilus |
| 1.17.1.9 | 25°C, glycine buffer, pH 10.5, 30 days, complete loss of activity | Thermothelomyces thermophilus |
| 1.17.1.9 | 4°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.9 | CO2 + NADH | - |
Thermothelomyces thermophilus | formate + NAD+ | - |
r | |
| 1.17.1.9 | CO2 + NADH | - |
Thermothelomyces thermophilus ATCC 42464 | formate + NAD+ | - |
r | |
| 1.17.1.9 | formate + NAD+ | - |
Thermothelomyces thermophilus | CO2 + NADH + H+ | - |
r | |
| 1.17.1.9 | formate + NAD+ | - |
Thermothelomyces thermophilus ATCC 42464 | CO2 + NADH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.1.9 | ? | x * 42000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.1.9 | FDH | - |
Thermothelomyces thermophilus |
| 1.17.1.9 | NAD+-dependent formate dehydrogenase | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 35 | - |
- |
Thermothelomyces thermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 25 | 49 | about 78% activity at 25°C, 100% activity at 35°C, about 90% activity at 45°C, about 67% activity at 47°C, and about 40% activity at 49°C. No activity at 65°C | Thermothelomyces thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 25 | 48 | melting temperature is 48°C. The enzyme remains stable for up to 30 min at 25°C | Thermothelomyces thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.03 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.1 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.3 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.76 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 5.5 | 6.5 | the enzyme displays a sharp pH optimum for the reaction with sodium bicarbonate at pH pH 5.5-6.0 | Thermothelomyces thermophilus |
| 1.17.1.9 | 10.5 | - |
the enzyme displays a pH optimum for the conversion of formate anion to carbon dioxide at pH pH 10.5 | Thermothelomyces thermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.9 | NAD+ | - |
Thermothelomyces thermophilus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.17.1.9 | Thermothelomyces thermophilus | isoelectric focusing | - |
6.2 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.04 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.17 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.23 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.63 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
