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Literature summary extracted from

  • Jaiswal, N.; Pandey, V.; Dwivedi, U.
    Purification of a thermostable laccase from Leucaena leucocephala using a copper alginate entrapment approach and the application of the laccase in dye decolorization (2014), Process Biochem., 49, 1196-1204 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents Leucaena leucocephala

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Benzene 1 h, 20% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 chloroform 1 h, 20% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 Co2+ 1 mM, 63% inhibition Leucaena leucocephala
1.10.3.2 dimethyl sulfoxide 1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 dithiothreitol 5 mM, 81% inhibition Leucaena leucocephala
1.10.3.2 EDTA 1 mM, 95% inhibition Leucaena leucocephala
1.10.3.2 ethanol 1 h, 20% loss of activity by 20% (v/v), 45% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 Hg2+ 1 mM, 50% inhibition Leucaena leucocephala
1.10.3.2 Isopropanol 1 h, 40% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 methanol 1 h, 20% loss of activity by 20% (v/v), 60% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 N,N-Dimethylformamide 1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v) Leucaena leucocephala
1.10.3.2 SDS 10 mM, 89% inhibition Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 1.24
-
 catechol pH 7.0 37°C Leucaena leucocephala
1.10.3.2 1.92
-
 hydroquinone pH 7.0 37°C Leucaena leucocephala
1.10.3.2 6.86
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.0 37°C Leucaena leucocephala

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ 1 mM, 1.3fold activation Leucaena leucocephala
1.10.3.2 Cd2+ 5 mM, 1.9fold activation Leucaena leucocephala
1.10.3.2 Cu2+ 1 mM, 1.3fold activation Leucaena leucocephala
1.10.3.2 Fe2+ 1 mM, 1.9fold activation Leucaena leucocephala
1.10.3.2 Mn2+ 10 mM, 4.3fold activation Leucaena leucocephala
1.10.3.2 Na+ 1 mM, 1.4fold activation Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 220000
-
 gel filtration Leucaena leucocephala

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Leucaena leucocephala
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Leucaena leucocephala

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 leaf
-
 Leucaena leucocephala
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 58.5
-
 pH 7.0 37°C Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Leucaena leucocephala ?
-
 ?
1.10.3.2 catechol + O2
-
 Leucaena leucocephala ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Leucaena leucocephala ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 heterodimer 1 * 100000 + 1 * 120000, SDS-PAGE Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 80
-
-
 Leucaena leucocephala

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 50 90 rapid increase in laccase activity from 50°C to 80°C followed by a decline inlaccase activity of approximately 26% at 90°C Leucaena leucocephala

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.042
-
 catechol pH 7.0 37°C Leucaena leucocephala
1.10.3.2 0.058
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.0 37°C Leucaena leucocephala
1.10.3.2 0.23
-
 hydroquinone pH 7.0 37°C Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 7
-
-
 Leucaena leucocephala

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 60% of maximal activity Leucaena leucocephala

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6 9 the enzyme retains more than 80% of its activity at within 24 h at 37°C Leucaena leucocephala

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.034
-
 catechol pH 7.0 37°C Leucaena leucocephala
1.10.3.2 0.12
-
 hydroquinone pH 7.0 37°C Leucaena leucocephala
1.10.3.2 118.28
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.0 37°C Leucaena leucocephala