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Literature summary extracted from
- New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase (1999), Proc. Natl. Acad. Sci. USA, 96, 10045-10050 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.B13 | 0.001 | - |
coniferaldehyde | pH 7.8, 30°C | Arabidopsis thaliana |  |
| 1.14.14.B13 | 0.003 | - |
coniferyl alcohol | pH 7.8, 30°C | Arabidopsis thaliana | |
| 1.14.14.B13 | 1 | - |
ferulate | pH 7.8, 30°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.B13 | 58000 | - |
SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.B13 | coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.B13 | Arabidopsis thaliana | Q42600 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.B13 | stem | flowering plant stem | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.B13 | coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.B13 | coniferyl alcohol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 5-hydroxyconiferyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.B13 | ferulate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 5-hydroxyferulate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.B13 | CYP84A1 | - |
Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.B13 | cytochrome P450 | heme-thiolate (P-450) monooxygenase | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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