Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hugo, M.; Martinez, A.; Trujillo, M.; Estrada, D.; Mastrogiovanni, M.; Linares, E.; Augusto, O.; Issoglio, F.; Zeida, A.; Estrin, D.A.; Heijnen, H.F.; Piacenza, L.; Radi, R.
    Kinetics, subcellular localization, and contribution to parasite virulence of a Trypanosoma cruzi hybrid type A heme peroxidase (TcAPx-CcP) (2017), Proc. Natl. Acad. Sci. USA, 114, E1326-E1335 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11
-
 Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.11 W233F complete loss of cytochrome c-dependent activity Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.029
-
 cytochrome c pH 7.4, 25°C Trypanosoma cruzi
1.11.1.11 0.19
-
 L-ascorbate pH 7.4, 25°C Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 mitochondrion enzyme is closely associated with mitochondrial membranes Trypanosoma cruzi 5739
-
1.11.1.11 plasma membrane enzyme is associated with plasma membrane Trypanosoma cruzi 5886
-

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Trypanosoma cruzi Q8I1N3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+
-
 Trypanosoma cruzi 2 monodehydroascorbate + 2 H2O
-
 ?
1.11.1.11 cytochrome c + H2O2
-
 Trypanosoma cruzi ? + H2O
-
 ?
1.11.1.11 additional information reaction includes formation of a compound I-like product, characteristic of the generation of a tryptophanyl radical-cation at residue W233. In addition, formation of a C222-derived radical is observed. electron transfer between Trp233 and Cys222 is possible and likely to participate in the catalytic cycle Trypanosoma cruzi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 APX
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
1.11.1.11 physiological function overexpressing strains are significantly more infective to macrophages and cardiomyocytes, as well as in the mouse model of Chagas disease than wild-type Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.11 35
-
 L-ascorbate pH 7.4, 25°C Trypanosoma cruzi
1.11.1.11 210
-
 cytochrome c pH 7.4, 25°C Trypanosoma cruzi